Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CDK5RAP3 antibody [EPR11293] - BSA and Azide free (ab249450)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11293] to CDK5RAP3 - BSA and Azide free
  • Suitable for: ICC, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-CDK5RAP3 antibody [EPR11293] - BSA and Azide free
    See all CDK5RAP3 primary antibodies

  • Description

    Rabbit monoclonal [EPR11293] to CDK5RAP3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, WBmore details
    Unsuitable for: Flow Cyt or IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human CDK5RAP3. The exact sequence is proprietary.
    Database link: Q96JB5

  • General notes

    Ab249450 is the carrier-free version of ab168353. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab249450 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-CDK5RAP3 antibody [EPR11293] - BSA and Azide free (ab249450)
    Western blot - Anti-CDK5RAP3 antibody [EPR11293] - BSA and Azide free (ab249450)
    All lanes : Anti-CDK5RAP3 antibody [EPR11293] (ab168353) at 1/1000 dilution

    Lane 1 : 293T (Human embryonic kidney epithelial cell) cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cell lysate
    Lane 4 : A431 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 57 kDa



    This data was developed using ab168353, the same antibody clone in a different buffer formulation.

  • Immunocytochemistry - Anti-CDK5RAP3 antibody [EPR11293] - BSA and Azide free (ab249450)
    Immunocytochemistry - Anti-CDK5RAP3 antibody [EPR11293] - BSA and Azide free (ab249450)
    This data was developed using ab168353, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of U87-MG cells labeling CDK5RAP3 with ab168353 at 1/50 dilution.
  • Western blot - Anti-CDK5RAP3 antibody [EPR11293] - BSA and Azide free (ab249450)
    Western blot - Anti-CDK5RAP3 antibody [EPR11293] - BSA and Azide free (ab249450)
    Anti-CDK5RAP3 antibody [EPR11293] (ab168353) at 1/1000 dilution + immunoprecipitation pellet from 293T cell lysate

    Secondary
    HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG

    Predicted band size: 57 kDa



    This data was developed using ab168353, the same antibody clone in a different buffer formulation.

     

  • Anti-CDK5RAP3 antibody [EPR11293] - BSA and Azide free (ab249450)
    Anti-CDK5RAP3 antibody [EPR11293] - BSA and Azide free (ab249450)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

© 2021 Astana Biomed Group LLP. All rights reserved.