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Astana Biomed Group, an authorized Abcam distributor in Central Asia

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Cancer Cell cycle Kinases/phosphatases Cdks

Anti-Cdk6 (phospho Y13) antibody (ab131439)

Anti-Cdk6 (phospho Y13) antibody (ab131439)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to Cdk6 (phospho Y13)
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Cdk6 (phospho Y13) antibody
    See all Cdk6 primary antibodies
  • Description

    Rabbit polyclonal to Cdk6 (phospho Y13)
  • Host species

    Rabbit
  • Specificity

    ab131439 detects endogenous levels of Cdk6 only when phosphorylated at tyrosine 13.
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic phosphopeptide conjugated to KLH, derived from around the phosphorylation site of Tyrosine 13 (

    Q-Q-Y(p)-E-C

    ) of Human Cdk6 (NP_001138778.1).
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • 293 cell extract treated with Hydroxyurea; HeLa cells; Human breast carcinoma tissue
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride

    PBS without Mg2+ and Ca2+
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab131439 was purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Kinases/Phosphatases
    • Cdks
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Kinases/Phosphatases
    • Cdks
    • Cancer
    • Cell cycle
    • Kinases/phosphatases
    • Cdks

Images

  • Western blot - Anti-Cdk6 (phospho Y13) antibody (ab131439)
    Western blot - Anti-Cdk6 (phospho Y13) antibody (ab131439)
    All lanes : Anti-Cdk6 (phospho Y13) antibody (ab131439) at 1/500 dilution

    Lane 1 : 293 cell extract untreated
    Lane 2 : 293 cell extract treated with Hydroxyurea

    Predicted band size: 37 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdk6 (phospho Y13) antibody (ab131439)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdk6 (phospho Y13) antibody (ab131439)
    Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling Cdk6 (phospho Y13) with ab131439 at 1/50 dilution. Right panel was preincubated with blocking peptide.
  • Immunocytochemistry/ Immunofluorescence - Anti-Cdk6 (phospho Y13) antibody (ab131439)
    Immunocytochemistry/ Immunofluorescence - Anti-Cdk6 (phospho Y13) antibody (ab131439)
    Immunofluorescent analysis of methanol-fixed HeLa cells labelling Cdk6 (phospho Y13) with ab131439 at 1/100 dilution.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-Cdk6 (phospho Y13) antibody (ab131439)

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