Antibodies, Proteins, Kits and Reagents for Life Science

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [BLR085G] to CDK1
Suitable for: ICC, IHC-P, WB, IP
Reacts with: Mouse, Human

Product name

Anti-CDK1 antibody [BLR085G]
See all CDK1 primary antibodies
Description

Rabbit monoclonal [BLR085G] to CDK1
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC	Human
IHC-P	Human
IP	Human
WB	Mouse Human

See all applications and species data

Immunogen

Synthetic peptide within Human CDK1 aa 1-50. The exact sequence is proprietary. NP_001777.1
Database link: P06493
Positive control
- IHC-P: Human breast carcinoma and prostate carcinoma tissue. ICC: MOLT4 cells. WB: HeLa, BW5147.3, F9, U-2 OS, K562, HEK-293T, A549, Jurkat, MCF7 and RKO whole cell lysate. IP: HeLa whole cell lysate.
General notes

This product is sold under License from Bethyl Laboratories, Inc

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.8
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline, 0.1% BSA
Concentration information loading...
Purity

Affinity purified
Purification notes

Purified from TCS.
Clonality

Monoclonal
Clone number

BLR085G
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK1 antibody (ab265590)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human breast carcinoma tissue labeling CDK1 with ab265590 at 1/100 dilution. Detection: DAB.
Western blot - Anti-CDK1 antibody (ab265590)

All lanes : Anti-CDK1 antibody [BLR085G] (ab265590) at 1/1000 dilution

Lane 1 : HeLa whole cell lysate
Lane 2 : U-2 OS whole cell lysate
Lane 3 : K562 whole cell lysate
Lane 4 : HEK-293T whole cell lysate
Lane 5 : A549 whole cell lysate
Lane 6 : Jurkat whole cell lysate
Lane 7 : MCF7 whole cell lysate
Lane 8 : RKO whole cell lysate

Lysates/proteins at 50 µg per lane.

Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG

Predicted band size: 34 kDa

Exposure time: 30 seconds

Cells prepared using NETN lysis buffer.
Immunocytochemistry - Anti-CDK1 antibody (ab265590)

Immunocytochemical analysis of formalin-fixed, paraffin-embedded molt4 cells labeling CDK1 with ab265590 at 1/100 dilution. Detection: DAB.
Western blot - Anti-CDK1 antibody (ab265590)

All lanes : Anti-CDK1 antibody [BLR085G] (ab265590) at 1/1000 dilution

Lane 1 : BW 5147.3 whole cell lysate
Lane 2 : F9 whole cell lysate

Lysates/proteins at 50 µg per lane.

Secondary
All lanes : HRP conjugated goat anti-rabbit IgG

Predicted band size: 34 kDa

Exposure time: 3 minutes

Cells prepared using NETN lysis buffer.
Immunoprecipitation - Anti-CDK1 antibody (ab265590)

CDK1 was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab265590 at 20 µl/mg lysate. Western blot was performed from the immunoprecipitate using ab265590 at 1/1000 dilution.

Lane 1: ab265590 IP in HeLa whole cell lysate.
Lane 2: Control IgG.

Exposure time: 10 secs.

Cells prepared using NETN lysis buffer.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK1 antibody (ab265590)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human prostate carcinoma tissue labeling CDK1 with ab265590 at 1/100 dilution. Detection: DAB.
Anti-CDK1 antibody [BLR085G] (ab265590)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com