Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸

Order now and get it on Tuesday March 02, 2021

Anti-CDK1 (phospho T161) antibody [EPR19546] (ab201008)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR19546] to CDK1 (phospho T161)
Suitable for: WB, Dot blot, IHC-P, IP, ICC/IF
Reacts with: Mouse, Rat, Human

Product name

Anti-CDK1 (phospho T161) antibody [EPR19546]
See all CDK1 primary antibodies
Description

Rabbit monoclonal [EPR19546] to CDK1 (phospho T161)
Host species

Rabbit
Specificity

ab201008 also recognizes CDK2 (phospho T160) and CDK3 (phospho T160).

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Rat Human
IP	Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HeLa treated with UV for 90 minutes, HeLa treated with UV for 90 minutes then with alkaline phosphatase for 1 hour, C6, C6 treated with alkaline phosphatase for 1 hour and NIH/3T3 whole cell lysate. IHC-P: Human tonsil and cervix cancer tissues; Rat testis tissue. ICC/IF: HeLa cells. IP: HeLa treated with 25J/m2 UV for 1-hour whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR19546
Isotype

IgG
Research areas

Western blot - Anti-CDK1 (phospho T161) antibody [EPR19546] (ab201008)

All lanes : Anti-CDK1 (phospho T161) antibody [EPR19546] (ab201008) at 1/1000 dilution

Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HeLa treated with UV for 90 minutes whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 34 kDa
Observed band size: 28,34 kDa
why is the actual band size different from the predicted?

Exposure time: 30 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
Dot Blot - Anti-CDK1 (phospho T161) antibody [EPR19546] (ab201008)

Dot blot analysis of CDK1 (phospho T161) labeled with ab201008 at 1/1000 dilution.

Lane 1: CDK1 (phospho T161) phospho peptide.
Lane 2: CDK1 non-phospho peptide.

Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody.

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure time: 3 minutes.
Dot Blot - Anti-CDK1 (phospho T161) antibody [EPR19546] (ab201008)

Dot blot analysis of CDK2 (phospho T160) labeled with ab201008 at 1/1000 dilution.

Lane 1: CDK2 (phospho T160) phospho peptide.
Lane 2: CDK2 non-phospho peptide.

Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody.

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure time: 3 minutes.
Dot Blot - Anti-CDK1 (phospho T161) antibody [EPR19546] (ab201008)

Dot blot analysis of CDK3 (phospho T160) labeled with ab201008 at 1/1000 dilution.

Lane 1: CDK3 (phospho T160) phospho peptide.
Lane 2: CDK3 non-phospho peptide.

Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody.

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure time: 3 minutes.
Western blot - Anti-CDK1 (phospho T161) antibody [EPR19546] (ab201008)

All lanes : Anti-CDK1 (phospho T161) antibody [EPR19546] (ab201008) at 1/1000 dilution

Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HeLa treated with UV for 90 minutes whole cell lysate
Lane 3 : HeLa treated with UV for 90 minutes then with alkaline phosphatase for 1-hour whole cell lysate
Lane 4 : HeLa treated with UV for 90 minutes then with alkaline phosphatase overnight whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 34 kDa
Observed band size: 28,34 kDa why is the actual band size different from the predicted?

Exposure time: 15 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-CDK1 (phospho T161) antibody [EPR19546] (ab201008)

All lanes : Anti-CDK1 (phospho T161) antibody [EPR19546] (ab201008) at 1/1000 dilution

Lane 1 : C6 (rat glial tumor cell line) whole cell lysate
Lane 2 : C6 treated with alkaline phosphatase for 1 hour whole cell lysate
Lane 3 : C6 treated with alkaline phosphatase overnight whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 34 kDa
Observed band size: 28,34 kDa why is the actual band size different from the predicted?

Exposure time: 10 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-CDK1 (phospho T161) antibody [EPR19546] (ab201008)

All lanes : Anti-CDK1 (phospho T161) antibody [EPR19546] (ab201008) at 1/1000 dilution

Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lane 2 : C6 (rat glial tumor cell line) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 34 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?

Exposure time: 3 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK1 (phospho T161) antibody [EPR19546] (ab201008)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CDK1 (phospho T161) with ab201008 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus and weak cytoplasm staining of germinal center from human tonsil is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK1 (phospho T161) antibody [EPR19546] (ab201008)

Immunohistochemical analysis of paraffin-embedded human cervix cancer tissue labeling CDK1 (phospho T161) with ab201008 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus and cytoplasm staining of cancer cells from human cervix cancer is observed [PMID: 15623629]. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK1 (phospho T161) antibody [EPR19546] (ab201008)

Immunohistochemical analysis of paraffin-embedded rat testis tissue labeling CDK1 (phospho T161) with ab201008 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus and cytoplasm staining of rat testis is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-CDK1 (phospho T161) antibody [EPR19546] (ab201008)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling CDK1 (phospho T161) with ab201008 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing specific signal in M phase cells. The signal decreased after treatment with lambda protein phosphatase 31°C for 5 hours.

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-Alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.
Immunoprecipitation - Anti-CDK1 (phospho T161) antibody [EPR19546] (ab201008)

CDK1 (phospho T161) was immunoprecipitated from 0.35mg of HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab201008 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab201008 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1: HeLa treated with 25J/m2 UV for 1 hour whole cell lysate 10 µg (Input).
Lane 2: ab201008 IP in HeLa treated with 25J/m2 UV for 1 hour whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab201008 in HeLa treated with 25J/m2 UV for 1 hour whole cell lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 3 minutes.
Anti-CDK1 (phospho T161) antibody [EPR19546] (ab201008)

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