Antibodies, Proteins, Kits and Reagents for Life Science

345 091 ₸ Product size

100 µl 345 091 ₸ 10 µl 127 315 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP715Y] to CDK5
Suitable for: WB, IHC-P, Flow Cyt, IP, ICC/IF
Knockout validated
Reacts with: Mouse, Human

Product name

Anti-CDK5 antibody [EP715Y]
See all CDK5 primary antibodies
Description

Rabbit monoclonal [EP715Y] to CDK5
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human Cdk5 aa 250 to the C-terminus (C terminal). The exact sequence is proprietary.
Positive control
- WB: HeLa whole cell lysate (ab150035). IHC-P: Human brain tissue. Flow Cyt: HeLa cells. IHC-Fr: Chicken midbrain tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.05% BSA, 49% PBS
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EP715Y
Isotype

IgG
Research areas

Western blot - Anti-Cdk5 antibody [EP715Y] (ab40773)

All lanes : Anti-CDK5 antibody [EP715Y] (ab40773) at 1/2000 dilution

Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : CDK5 knockout HAP1 whole cell lysate
Lane 3 : SHSY5Y whole cell lysate
Lane 4 : MCF7 whole cell lysate

Lysates/proteins at 20 µg per lane.

Predicted band size: 33 kDa
Observed band size: 33 kDa

Lanes 1 - 4: Merged signal (red and green). Green - ab40773 observed at 33 kDa. Red - loading control, ab130007, observed at 130 kDa.

ab40773 was shown to specifically react with Cdk5 in wild-type HAP1 cells as signal was lost in CDK5 knockout cells. Wild-type and CDK5 knockout samples were subjected to SDS-PAGE. Ab40773 and ab130007 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/2000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-Cdk5 antibody [EP715Y] (ab40773)

Anti-CDK5 antibody [EP715Y] (ab40773) at 1/10000 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate

Predicted band size: 33 kDa
Observed band size: 33 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Cdk5 antibody [EP715Y] (ab40773)

ab40773 staining Cdk5 in the SH-SY5Y cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody (1/300). a150077 was used as the secondary antibody (1/500) and nuclei stained with DAPI.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdk5 antibody [EP715Y] (ab40773)

Paraffin-embedded human brain tissue stained for Cdk5 with ab40773 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cytometry - Anti-Cdk5 antibody [EP715Y] (ab40773)

Flow cytometry analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labelling Cdk5 with ab40773 (pink). Negative control Rabbit IgG (green).
Immunoprecipitation - Anti-Cdk5 antibody [EP715Y] (ab40773)

ab40773 at 1/50 immunoprecipitating Cdk5 in HeLa whole cell lysate.
For western blotting, the primary antibody was used at a dilution of 1/1000 and a HRP-conjugated goat anti-rabbit IgG was used as the secondary antibody (1/1000).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Anti-CDK5 antibody [EP715Y] (ab40773)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com