Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CDK2 (phospho T160) + CDK1 (phospho T161) antibody [EPR17621] - BSA and Azide free (ab250675)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17621] to CDK2 (phospho T160) + CDK1 (phospho T161) - BSA and Azide free
  • Suitable for: Dot blot, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-CDK2 (phospho T160) + CDK1 (phospho T161) antibody [EPR17621] - BSA and Azide free
    See all CDK2 (phospho T160) + CDK1 (phospho T161) primary antibodies

  • Description

    Rabbit monoclonal [EPR17621] to CDK2 (phospho T160) + CDK1 (phospho T161) - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab250675 is the carrier-free version of ab183554. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250675 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-CDK2 (phospho T160) + CDK1 (phospho T161) antibody [EPR17621] - BSA and Azide free (ab250675)
    Western blot - Anti-CDK2 (phospho T160) + CDK1 (phospho T161) antibody [EPR17621] - BSA and Azide free (ab250675)
    All lanes : Anti-CDK2 (phospho T160) + CDK1 (phospho T161) antibody [EPR17621] (ab183554) at 1/1000 dilution

    Lane 1 : Untreated HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate treated with 40 ng/ml nocodazole for 24 hours

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 34 kDa
    Observed band size: 34 kDa


    Exposure time: 3 minutes


    This data was developed using ab183554, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Dot Blot - Anti-CDK2 (phospho T160) + CDK1 (phospho T161) antibody [EPR17621] - BSA and Azide free (ab250675)
    Dot Blot - Anti-CDK2 (phospho T160) + CDK1 (phospho T161) antibody [EPR17621] - BSA and Azide free (ab250675)
    This data was developed using ab183554, the same antibody clone in a different buffer formulation.Dot blot analysis of CDK2 (phospho T160) phospho peptide (Lane 1) and CDK2 non peptide (Lane 2) using ab183554 at 1/1000 dilution detecting CDK2 (phospho T160) phospho peptide only. A Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 was used as secondary antibody.Blocking and diluting buffer: 5% NFDM/TBST.Exposure time: 3 minutes.
  • Dot Blot - Anti-CDK2 (phospho T160) + CDK1 (phospho T161) antibody [EPR17621] - BSA and Azide free (ab250675)
    Dot Blot - Anti-CDK2 (phospho T160) + CDK1 (phospho T161) antibody [EPR17621] - BSA and Azide free (ab250675)
    This data was developed using ab183554, the same antibody clone in a different buffer formulation.Dot blot analysis of CDK1 (phospho T161) phospho peptide (Lane 1) and CDK1 non peptide (Lane 2) using ab183554 at 1/1000 dilution detecting CDK1 (phospho T161) phospho peptide only. A Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 was used as secondary antibody.Blocking and diluting buffer: 5% NFDM/TBST.Exposure time: 3 minutes.
  • Anti-CDK2 (phospho T160) + CDK1 (phospho T161) antibody [EPR17621] - BSA and Azide free (ab250675)
    Anti-CDK2 (phospho T160) + CDK1 (phospho T161) antibody [EPR17621] - BSA and Azide free (ab250675)

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