Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR22815-112] - BSA and Azide free (ab256475)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22815-112] to Glutathione Peroxidase 3/GPx-3 - BSA and Azide free
  • Suitable for: WB, IHC-P, IHC-Fr
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR22815-112] - BSA and Azide free
    See all Glutathione Peroxidase 3/GPx-3 primary antibodies

  • Description

    Rabbit monoclonal [EPR22815-112] to Glutathione Peroxidase 3/GPx-3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, IHC-Frmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Mouse, Rat

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse and rat kidney tissue lysate. IHC-P: Mouse and rat kidney and lung tissue. IHC-Fr: Mouse and rat kidney tissue.

  • General notes

    Ab256475 is the carrier-free version of ab256470. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab256475 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Frozen sections) - Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR22815-112] - BSA and Azide free (ab256475)
    Immunohistochemistry (Frozen sections) - Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR22815-112] - BSA and Azide free (ab256475)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat kidney tissue labeling Glutathione Peroxidase 3/GPx-3 with ab256470 at 1/100 (4.80 μg/ml) dilution followed by ab150077 AlexaFluor® 488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution. The nuclear counterstain was DAPI (Blue). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Positive staining on rat kidney (PMID: 21493731) is observed.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was ab150077 AlexaFluor® 488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256470).

  • Immunohistochemistry (Frozen sections) - Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR22815-112] - BSA and Azide free (ab256475)
    Immunohistochemistry (Frozen sections) - Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR22815-112] - BSA and Azide free (ab256475)

    Immunohistochemical analysis of 4% PFA fixed, 0.2% Triton X-100 permeabilized frozen mouse kidney tissue labeling Glutathione Peroxidase 3/GPx-3 with ab256470 at 1/100 (4.80 μg/ml) dilution followed by ab150077 AlexaFluor® 488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution. The nuclear counterstain was DAPI (Blue). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Positive staining on mouse kidney (PMID: 21493731) is observed.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was ab150077 AlexaFluor® 488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256470).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR22815-112] - BSA and Azide free (ab256475)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR22815-112] - BSA and Azide free (ab256475)

    Immunohistochemical analysis of paraffin-embedded rat lung tissue labeling Glutathione Peroxidase 3/GPx-3 with ab256470 at 1/500 dilution (0.96µg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat lung (PMID: 22355371) is observed. The section was incubated with ab256470 for 15 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256470).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR22815-112] - BSA and Azide free (ab256475)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR22815-112] - BSA and Azide free (ab256475)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling Glutathione Peroxidase 3/GPx-3 with ab256470 at 1/500 dilution (0.96µg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat kidney (PMID: 21493731, 27435875) is observed. The section was incubated with ab256470 for 15 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256470).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR22815-112] - BSA and Azide free (ab256475)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR22815-112] - BSA and Azide free (ab256475)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling Glutathione Peroxidase 3/GPx-3 with ab256470 at 1/500 dilution (0.96µg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse lung (PMID: 22355371) is observed. The section was incubated with ab256470 for 15 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256470).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR22815-112] - BSA and Azide free (ab256475)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR22815-112] - BSA and Azide free (ab256475)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Glutathione Peroxidase 3/GPx-3 with ab256470 at 1/500 dilution (0.96µg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse kidney (PMID: 21493731, 27435875) is observed. The section was incubated with ab256470 for 15 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256470).

  • Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR22815-112] - BSA and Azide free (ab256475)
    Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR22815-112] - BSA and Azide free (ab256475)

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