Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Myosin, smooth muscle heavy chain 1 and 2 antibody [EPR5335] - BSA and Azide free (ab247958)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5335] to smooth muscle Myosin heavy chain 11 - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5335] - BSA and Azide free
    See all smooth muscle Myosin heavy chain 11 primary antibodies

  • Description

    Rabbit monoclonal [EPR5335] to smooth muscle Myosin heavy chain 11 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human fetal artery, bladder and mouse spleen lysates IHC: Human colon and kidney tissues

  • General notes

    ab247958 is the carrier-free version of ab124679.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

Properties

Images

  • Western blot - Anti-Myosin, smooth muscle heavy chain 1 and 2 antibody [EPR5335] - BSA and Azide free (ab247958)
    Western blot - Anti-Myosin, smooth muscle heavy chain 1 and 2 antibody [EPR5335] - BSA and Azide free (ab247958)
    Anti-Myosin, smooth muscle heavy chain 1 and 2 antibody [EPR5335] (ab124679) at 1/20000 dilution (purified) + mouse spleen at 20 µg

    Secondary
    HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 227 kDa
    Observed band size: 250 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab124679, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Myosin, smooth muscle heavy chain 1 and 2 antibody [EPR5335] - BSA and Azide free (ab247958)
    Western blot - Anti-Myosin, smooth muscle heavy chain 1 and 2 antibody [EPR5335] - BSA and Azide free (ab247958)
    Anti-Myosin, smooth muscle heavy chain 1 and 2 antibody [EPR5335] (ab124679) at 1/50000 dilution (purified) + human fetal artery lysate at 10 µg

    Secondary
    HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution

    Predicted band size: 227 kDa
    Observed band size: 250 kDa why is the actual band size different from the predicted?



    This data was developed using ab124679, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin, smooth muscle heavy chain 1 and 2 antibody [EPR5335] - BSA and Azide free (ab247958)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin, smooth muscle heavy chain 1 and 2 antibody [EPR5335] - BSA and Azide free (ab247958)

    This data was developed using ab124679, the same antibody clone in a different buffer formulation.

    Immunohistochemical staining of paraffin embedded human smooth muscle with purified ab124679 at a working dilution of 1/500. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin, smooth muscle heavy chain 1 and 2 antibody [EPR5335] - BSA and Azide free (ab247958)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin, smooth muscle heavy chain 1 and 2 antibody [EPR5335] - BSA and Azide free (ab247958)
    This data was developed using ab124679, the same antibody clone in a different buffer formulation.Immunohistochemical staining of paraffin embedded human skeletal muscle with purified ab124679 at a working dilution of 1/500. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin, smooth muscle heavy chain 1 and 2 antibody [EPR5335] - BSA and Azide free (ab247958)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin, smooth muscle heavy chain 1 and 2 antibody [EPR5335] - BSA and Azide free (ab247958)
    This data was developed using ab124679, the same antibody clone in a different buffer formulation.Unpurified ab124679, at 1/100 dilution, staining Myosin, smooth muscle heavy chain 1 and 2 in paraffin-embedded Human colon tissue by Immunohistochemistry. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin, smooth muscle heavy chain 1 and 2 antibody [EPR5335] - BSA and Azide free (ab247958)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin, smooth muscle heavy chain 1 and 2 antibody [EPR5335] - BSA and Azide free (ab247958)
    This data was developed using ab124679, the same antibody clone in a different buffer formulation.Unpurified ab124679, at 1/100 dilution, staining Myosin, smooth muscle heavy chain 1 and 2 in paraffin-embedded Human kidney tissue by Immunohistochemistry. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Western blot - Anti-Myosin, smooth muscle heavy chain 1 and 2 antibody [EPR5335] - BSA and Azide free (ab247958)
    Western blot - Anti-Myosin, smooth muscle heavy chain 1 and 2 antibody [EPR5335] - BSA and Azide free (ab247958)
    All lanes : Anti-Myosin, smooth muscle heavy chain 1 and 2 antibody [EPR5335] (ab124679) at 1/10000 dilution (unpurified)

    Lane 1 : Human fetal artery lysate
    Lane 2 : Human bladder lysate
    Lane 3 : Mouse spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size: 227 kDa
    Observed band size: 250 kDa why is the actual band size different from the predicted?



    This data was developed using ab124679, the same antibody clone in a different buffer formulation.

  • OI-RD Scanning - Anti-Myosin, smooth muscle heavy chain 1 and 2 antibody [EPR5335] - BSA and Azide free (ab247958)
    OI-RD Scanning - Anti-Myosin, smooth muscle heavy chain 1 and 2 antibody [EPR5335] - BSA and Azide free (ab247958)
    This data was developed using ab124679, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-Myosin, smooth muscle heavy chain 1 and 2 antibody [EPR5335] - BSA and Azide free (ab247958)
    Anti-Myosin, smooth muscle heavy chain 1 and 2 antibody [EPR5335] - BSA and Azide free (ab247958)

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