Anti-MYL9 (phospho T18 + S19) antibody [18HCLC] (ab277790)
![Anti-MYL9 (phospho T18 + S19) antibody [18HCLC] (ab277790)](https://www.abcam.com/ps/products/277/ab277790/Images/ab277790-2-antiPhosphoMYL9T18S19recombinantpolyclonalantibody18hclcWB.jpg "Anti-MYL9 (phospho T18 + S19) antibody [18HCLC] (ab277790)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit recombinant multiclonal [18HCLC] to MYL9 (phospho T18 + S19)
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
Overview
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Product name
Anti-MYL9 (phospho T18 + S19) antibody [18HCLC]
See all MYL9 primary antibodies -
Description
Rabbit recombinant multiclonal [18HCLC] to MYL9 (phospho T18 + S19) -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide aa 1-100 (phospho T18 + S19).
Database link: P24844 -
Positive control
- WB: human HeLa cells treated with Calyculin A; human U-2 OS cells treated with Calyculin A; mouse C2C12 cells treated with Calyculin A ICC/IF: C2C12 cells
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General notes
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains.
Recombinant multiclonal antibodies offer the sensitivity of polyclonal antibodies by recognising multiple epitopes, along with consistency of a recombinant antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide
Constituent: 99.91% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Recombinant Multiclonal -
Clone number
18HCLC -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-MYL9 antibody (ab277790)All lanes : Anti-MYL9 (phospho T18 + S19) antibody [18HCLC] (ab277790) at 2 µg/ml
Lane 1 : C2C12 (Mouse myoblast cell line) whole cell extract
Lane 2 : C2C12 (Mouse myoblast cell line) whole cell extract (treated with Calyculin A 50 ng for 20 minutes)
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract (treated with Calyculin A 50 ng for 20 minutes)
Lane 5 : U-2 OS (human bone osteosarcoma epithelial cell line) whole cell extract
Lane 6 : U-2 OS (human bone osteosarcoma epithelial cell line) whole cell extract (treated with Calyculin A 50 ng for 20 minutes)
Predicted band size: 20 kDa
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Immunocytochemistry - Anti-MYL9 antibody (ab277790)For immunofluorescence analysis, C2C12 cells were fixed and permeabilized for detection of endogenous Myosin regulatory light polypeptide 9 (pT18/pS19) using Myosin regulatory light polypeptide 9 (pT18/pS19) Recombinant Rabbit Multiclonal Antibody (ab277790, 2 μ/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (1/2000). Panel a) shows representative cells that were stained for detection and localization of Myosin regulatory light polypeptide 9 (pT18/pS19) protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI. Panel c) represents cytoskeletal F-actin staining using Alexa Fluor® 555 Rhodamine Phalloidin (1/300). Panel d) is a composite image of Panels a, b and c clearly demonstrating cytoplasmic localization of Myosin regulatory light polypeptide 9 (pT18/pS19). Panel e) shows loss of signal by competition with the Myosin regulatory light polypeptide 9 (pT18/pS19) peptide, demonstrating antibody specificity, and panel f) demonstrates no competition with the non-phosphorylated peptide. Panel μ) represents control cells with no primary antibody to assess background.
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![Anti-MYL9 antibody [18HCLC] (ab277790)](https://www.abcam.com/ps/products/277/ab277790/Images/ab277790-3-benefits-of-recombinant-antibodies.jpg)
Anti-MYL9 antibody [18HCLC] (ab277790)
