Anti-MYH2 antibody [EPR5280] (ab124937)
![Anti-MYH2 antibody [EPR5280] (ab124937)](https://www.abcam.com/ps/products/124/ab124937/Images/ab124937-138972-anti-myh2-antibody-epr5280-western-blot.jpg "Anti-MYH2 antibody [EPR5280] (ab124937)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5280] to MYH2
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-MYH2 antibody [EPR5280]
See all MYH2 primary antibodies -
Description
Rabbit monoclonal [EPR5280] to MYH2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseHuman
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Immunogen
Synthetic peptide within Human MYH2 aa 50-150. The exact sequence is proprietary.
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Positive control
- Mouse muscle, Rat muscle and Human trachea lysates
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 6.90 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR5280 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-MYH2 antibody [EPR5280] (ab124937)All lanes : Anti-MYH2 antibody [EPR5280] (ab124937) at 1/10000 dilution
Lane 1 : Mouse muscle lysate
Lane 2 : Rat muscle lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled Goat anti Rabbit IgG at 1/2000 dilution
Predicted band size: 223 kDa
Observed band size: 220 kDa why is the actual band size different from the predicted?
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![Western blot - Anti-MYH2 antibody [EPR5280] (ab124937)](https://www.abcam.com/ps/products/124/ab124937/Images/ab124937-138971-anti-myh2-antibody-epr5280-western-blot.jpg)
Western blot - Anti-MYH2 antibody [EPR5280] (ab124937)Anti-MYH2 antibody [EPR5280] (ab124937) at 1/10000 dilution + Human trachea lysate at 10 µg
Secondary
HRP labelled Goat anti Rabbit IgG at 1/2000 dilution
Predicted band size: 223 kDa
Observed band size: 220 kDa why is the actual band size different from the predicted?
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![OI-RD Scanning - Anti-MYH2 antibody [EPR5280] (ab124937)](https://www.abcam.com/ps/products/124/ab124937/Images/ab124937-138970-anti-myh2-antibody-epr5280.jpg)
OI-RD Scanning - Anti-MYH2 antibody [EPR5280] (ab124937) -
![Anti-MYH2 antibody [EPR5280] (ab124937)](https://www.abcam.com/ps/products/124/ab124937/Images/ab124937-4-benefits-of-recombinant-antibodies.png)
Anti-MYH2 antibody [EPR5280] (ab124937)