Anti-Involucrin antibody [SY8] (ab20202)
![Anti-Involucrin antibody [SY8] (ab20202)](https://www.abcam.com/ps/products/20/ab20202/Images/Involucrin-Primary-antibodies-ab20202-1.jpg "Anti-Involucrin antibody [SY8] (ab20202)")
Key features and details
- Mouse monoclonal [SY8] to Involucrin
- Suitable for: IP, IHC-P, WB
- Reacts with: Human, Pig, Gorilla
- Isotype: IgG1
Overview
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Product name
Anti-Involucrin antibody [SY8]
See all Involucrin primary antibodies -
Description
Mouse monoclonal [SY8] to Involucrin -
Host species
Mouse -
Specificity
Keratinocytes of epidermis and other stratified squamous epithelia -
Tested applications
Suitable for: IP, IHC-P, WBmore details -
Species reactivity
Reacts with: Human, Pig, Gorilla -
Immunogen
Pure involucrin from human keratinocytes.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
SY8 -
Myeloma
P3x63 -
Isotype
IgG1 -
Light chain type
unknown -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Involucrin antibody [SY8] (ab20202)Ab20202 staining Human skin Staining is localised to the cytoplasm.
Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the DAKO 3-in-1 antigen retrieval buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
