Anti-RAC3 antibody [EPR6679(B)] (ab124943)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6679(B)] to RAC3
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Overview
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Product name
Anti-RAC3 antibody [EPR6679(B)]
See all RAC3 primary antibodies -
Description
Rabbit monoclonal [EPR6679(B)] to RAC3 -
Host species
Rabbit -
Specificity
ab124943 cross-reacts with Rac1, Rac2 and Cdc42 -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB Human -
Immunogen
Synthetic peptide within Human RAC3 aa 50-150. The exact sequence is proprietary.
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Positive control
- Human heart tissue, Human placenta tissue; JAR cells; Human fetal brain lysate, SH-SY5Y, JAR and BxPC-3 cell lysates
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 1.23 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR6679(B) -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-RAC3 antibody [EPR6679(B)] (ab124943) at 1/1000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : SH-SY5Y cell lysate
Lane 3 : JAR cell lysate
Lane 4 : BxPC-3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : standard HRP labelled goat anti-rabbit at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 21 kDa
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ab124943, at a 1/50 dilution, staining RAC3 in paraffin-embedded Human heart tissue by immunohistochemistry
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ab124943, at a 1/50 dilution, staining RAC3 in paraffin-embedded Human placenta tissue by immunohistochemistry
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ab124943, at a 1/50 dilution, staining RAC3 in JAR cells by immunofluorescence
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