Anti-SNAP23 antibody [EPR8539] (ab133703)
![Anti-SNAP23 antibody [EPR8539] (ab133703)](https://www.abcam.com/ps/products/133/ab133703/Images/ab133703-269768-anti-snap23-antibody-epr8539-western-blot.jpg "Anti-SNAP23 antibody [EPR8539] (ab133703)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8539] to SNAP23
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Human
Overview
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Product name
Anti-SNAP23 antibody [EPR8539]
See all SNAP23 primary antibodies -
Description
Rabbit monoclonal [EPR8539] to SNAP23 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide within Human SNAP23 aa 150-250 (C terminal). The exact sequence is proprietary.
Database link: O00161 -
Positive control
- Lysates of: fetal liver, HeLa, HepG2, BxPC-3
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 2.50 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR8539 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SNAP23 antibody [EPR8539] (ab133703)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: SNAP23 knockout HAP1 cell lysate (20 µg)
Lanes 1 - 2: Merged signal (red and green). Green - ab133703 observed at 25 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab133703 was shown to specifically react with SNAP23 when SNAP23 knockout samples were used. Wild-type and SNAP23 knockout samples were subjected to SDS-PAGE. ab133703 and ab8245 (loading control to GAPDH) were diluted at 1/500 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging. -
![Western blot - Anti-SNAP23 antibody [EPR8539] (ab133703)](https://www.abcam.com/ps/products/133/ab133703/Images/ab133703-147721-anti-snap23-antibody-epr8539-western-blot.jpg)
Western blot - Anti-SNAP23 antibody [EPR8539] (ab133703)All lanes : Anti-SNAP23 antibody [EPR8539] (ab133703) at 1/1000 dilution
Lane 1 : Fetal liver cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : BxPC-3 cell lysate
Predicted band size: 23 kDa
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![OI-RD Scanning - Anti-SNAP23 antibody [EPR8539] (ab133703)](https://www.abcam.com/ps/products/133/ab133703/Images/ab133703-147720-anti-snap23-antibody-epr8539.jpg)
OI-RD Scanning - Anti-SNAP23 antibody [EPR8539] (ab133703) -
![Anti-SNAP23 antibody [EPR8539] (ab133703)](https://www.abcam.com/ps/products/133/ab133703/Images/ab133703-4-benefits-of-recombinant-antibodies.png)
Anti-SNAP23 antibody [EPR8539] (ab133703)
