Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SNAP23 antibody [EPR8538] (ab131242)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8538] to SNAP23
  • Suitable for: Flow Cyt, WB, IHC-P, ICC/IF
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-SNAP23 antibody [EPR8538]
    See all SNAP23 primary antibodies

  • Description

    Rabbit monoclonal [EPR8538] to SNAP23

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human SNAP23 aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: O00161

  • Positive control

    • HeLa, Human placenta, BxPC-3, HepG2 and JAR cell lysates; Human colon tissue

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-SNAP23 antibody [EPR8538] (ab131242)
    Western blot - Anti-SNAP23 antibody [EPR8538] (ab131242)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: SNAP23 knockout HAP1 cell lysate (20 µg)
    Lane 3: HEK293 cell lysate (20 µg)
    Lane 4: Jurkat cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab131242 observed at 25 kDa. Red - loading control, ab8245, observed at 37kDa.
    ab131242 was shown to recognize SNAP23 when SNAP23 knockout samples were used, along with additional cross-reactive bands. Wild-type and SNAP23 knockout samples were subjected to SDS-PAGE. ab131242 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAP23 antibody [EPR8538] (ab131242)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAP23 antibody [EPR8538] (ab131242)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling SNAP23 with ab131242 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-SNAP23 antibody [EPR8538] (ab131242)
    Immunocytochemistry/ Immunofluorescence - Anti-SNAP23 antibody [EPR8538] (ab131242)

    Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) labeling SNAP23 with purified ab131242 at 1/500 dilution. Cells were fixed with 100% methanol. ab150077 Goat anti rabbit IgG (Alexa Fluor® 488) at 1/1000 was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.

  • Flow Cytometry - Anti-SNAP23 antibody [EPR8538] (ab131242)
    Flow Cytometry - Anti-SNAP23 antibody [EPR8538] (ab131242)

    Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling SNAP23 with unpurified ab131242 at 1/20 dilution(10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

  • Western blot - Anti-SNAP23 antibody [EPR8538] (ab131242)
    Western blot - Anti-SNAP23 antibody [EPR8538] (ab131242)
    All lanes : Anti-SNAP23 antibody [EPR8538] (ab131242) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Human placenta cell lysate
    Lane 3 : BxPC-3 cell lysate
    Lane 4 : HepG2 cell lysat
    Lane 5 : JAR cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 23 kDa

  • OI-RD Scanning - Anti-SNAP23 antibody [EPR8538] (ab131242)
    OI-RD Scanning - Anti-SNAP23 antibody [EPR8538] (ab131242)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-SNAP23 antibody [EPR8538] (ab131242)
    Anti-SNAP23 antibody [EPR8538] (ab131242)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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