Anti-PPAR gamma 1+2 antibody [A3409A] (ab41928)
![Anti-PPAR gamma 1+2 antibody [A3409A] (ab41928)](https://www.abcam.com/ps/products/41/ab41928/Images/ab41928-55360-anti-ppar-gamma-12-antibody-a3409a-chip-grade-immunohistochemistry.jpg "Anti-PPAR gamma 1+2 antibody [A3409A] (ab41928)")
Key features and details
- Mouse monoclonal [A3409A] to PPAR gamma 1+2
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG2a
Overview
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Product name
Anti-PPAR gamma 1+2 antibody [A3409A] -
Description
Mouse monoclonal [A3409A] to PPAR gamma 1+2 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P RatWB Mouse
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Immunogen
Baculovirus-expressed recombinant fragment, corresponding to amino acids 3-105 of Human PPAR gamma 1+2.
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Positive control
- IHC: Rat adipose cells and rat placenta. WB: Nuclear lysate of 3T3l-1 cells induced for adipocyte differentiation
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General notes
This product was changed from ascites to tissue culture supernatant on 3rd April 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.1% Sodium azide
Physiological saline. -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
A3409A -
Isotype
IgG2a -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPAR gamma 1+2 antibody [A3409A] (ab41928)
ab41928 staining PPAR gamma 1+2 in rat adiposoe cells (10 ug/mL) by immunohistochemistry, formalin-fixed paraffin embedded sections.
This image was generated using the ascites version of the product.
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![Western blot - Anti-PPAR gamma 1+2 antibody [A3409A] (ab41928)](https://www.abcam.com/ps/products/41/ab41928/Images/ab41928-55358-anti-ppar-gamma-12-antibody-a3409a-chip-grade-western-blot.jpg)
Western blot - Anti-PPAR gamma 1+2 antibody [A3409A] (ab41928)All lanes : Anti-PPAR gamma 1+2 antibody [A3409A] (ab41928) at 1 µg/ml
Lane 1 : Nuclear lysate of non induced
3T3l-1 cells
Lane 2 : Nuclear lysate of 3T3l-1 cells induced for adipocyte differentiation
Lysates/proteins at 20 µg per lane.
Predicted band size: 58 kDaTwo bands are visible: PPAR gamma 1 and PPAR gamma 2.
This image was generated using the ascites version of the product.
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![Immunoprecipitation - Anti-PPAR gamma 1+2 antibody [A3409A] (ab41928)](https://www.abcam.com/ps/products/41/ab41928/Images/ab41928-281001-anti-ppar-gamma-12-antibody-a3409a-chip-grade-immunoprecipitation.jpg)
Immunoprecipitation - Anti-PPAR gamma 1+2 antibody [A3409A] (ab41928)Lane 1: FLAG-M2
Lane 2: ab41928
Lane 3: ab41928
Lane 4: Control IgGImmunoprecipitation analysis using Fluorescence labeled PPAR gamma generated in vitro translation. Anti-PPAR gamma antibody (ab41928) is diluted with translation production and mixed with protein G Sepharose beads. It is then rotated at 4oC for 1 hour and precipitates are eluted with addition of SDS loading buffer. Samples are incubated for 10minutes at 70°C and analyzed with Fluorescence imager.
This image was generated using the ascites version of the product.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPAR gamma 1+2 antibody [A3409A] (ab41928)](https://www.abcam.com/ps/products/41/ab41928/Images/ab41928-55359-anti-ppar-gamma-12-antibody-a3409a-chip-grade-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPAR gamma 1+2 antibody [A3409A] (ab41928)ab41928 staining PPAR gamma 1+2 in rat plancenta(10 ug/mL) by immunohistochemistry, formalin-fixed paraffin embedded sections.
This image was generated using the ascites version of the product.
