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Astana Biomed Group, an authorized Abcam distributor in Central Asia

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Signal Transduction Protein Trafficking Vesicle Transport SNAPs & SNAREs

Anti-SNAP23 antibody (ab229085)

Anti-SNAP23 antibody (ab229085)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to SNAP23
  • Suitable for: WB, ICC/IF, IHC-P
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-SNAP23 antibody
    See all SNAP23 primary antibodies
  • Description

    Rabbit polyclonal to SNAP23
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Rhesus monkey
  • Immunogen

    Recombinant fragment within Human SNAP23 (internal sequence). The exact sequence is proprietary.
    Database link: O00161

  • Positive control

    • ICC/IF: DIV9 rat E18 primary cortical neurons; HeLa cells. WB: Mouse and rat brain extracts; A431 whole cell lysate. IHC-P: Human breast cancer tissue.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Secretory Vesicles
    • SNAPs & SNAREs
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Synapse marker

Images

  • Western blot - Anti-SNAP23 antibody (ab229085)
    Western blot - Anti-SNAP23 antibody (ab229085)
    All lanes : Anti-SNAP23 antibody (ab229085) at 1/500 dilution

    Lane 1 : Mouse brain extract
    Lane 2 : Rat brain extract

    Lysates/proteins at 50 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 23 kDa



    12% SDS-PAGE gel.

  • Immunocytochemistry/ Immunofluorescence - Anti-SNAP23 antibody (ab229085)
    Immunocytochemistry/ Immunofluorescence - Anti-SNAP23 antibody (ab229085)

    DIV9 rat E18 primary cortical neurons stained for SNAP23 (green) using ab229085 at 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.

    Red: beta Tubulin 3, a neuron cell marker, stained by beta Tubulin 3 antibody at 1/500 dilution.

    Blue: DAPI.

  • Immunocytochemistry/ Immunofluorescence - Anti-SNAP23 antibody (ab229085)
    Immunocytochemistry/ Immunofluorescence - Anti-SNAP23 antibody (ab229085)

    HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for SNAP23 (green) using ab229085 at 1/500 dilution in ICC/IF. Cells were fixed in methanol.

    Blue: Hoechst 33342 staining.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAP23 antibody (ab229085)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAP23 antibody (ab229085)

    Paraffin-embedded human breast cancer tissue stained for SNAP23 using ab229085 at 1/250 dilution in immunohistochemical analysis.

  • Western blot - Anti-SNAP23 antibody (ab229085)
    Western blot - Anti-SNAP23 antibody (ab229085)
    Anti-SNAP23 antibody (ab229085) at 1/500 dilution + A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg

    Predicted band size: 23 kDa



    12% SDS-PAGE gel.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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