Anti-SNAP23 antibody (ab229085)
Key features and details
- Rabbit polyclonal to SNAP23
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-SNAP23 antibody
See all SNAP23 primary antibodies -
Description
Rabbit polyclonal to SNAP23 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Rhesus monkey -
Immunogen
Recombinant fragment within Human SNAP23 (internal sequence). The exact sequence is proprietary.
Database link: O00161 -
Positive control
- ICC/IF: DIV9 rat E18 primary cortical neurons; HeLa cells. WB: Mouse and rat brain extracts; A431 whole cell lysate. IHC-P: Human breast cancer tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-SNAP23 antibody (ab229085) at 1/500 dilution
Lane 1 : Mouse brain extract
Lane 2 : Rat brain extract
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 23 kDa12% SDS-PAGE gel.
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DIV9 rat E18 primary cortical neurons stained for SNAP23 (green) using ab229085 at 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.
Red: beta Tubulin 3, a neuron cell marker, stained by beta Tubulin 3 antibody at 1/500 dilution.
Blue: DAPI.
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HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for SNAP23 (green) using ab229085 at 1/500 dilution in ICC/IF. Cells were fixed in methanol.
Blue: Hoechst 33342 staining.
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Paraffin-embedded human breast cancer tissue stained for SNAP23 using ab229085 at 1/250 dilution in immunohistochemical analysis.
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Anti-SNAP23 antibody (ab229085) at 1/500 dilution + A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg
Predicted band size: 23 kDa12% SDS-PAGE gel.