Anti-TLR2 antibody (ab213676)
Key features and details
- Rabbit polyclonal to TLR2
- Suitable for: IHC-P, WB, Flow Cyt
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-TLR2 antibody
See all TLR2 primary antibodies -
Description
Rabbit polyclonal to TLR2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt MouseHumanIHC-P RatWB Mouse
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Immunogen
Synthetic peptide within Human TLR2 aa 700 to the C-terminus conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: O60603 -
Positive control
- Rat ovary tissue; mouse spleen and Human A549 cells; rat brain and rat lung lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-TLR2 antibody (ab213676)All lanes : Anti-TLR2 antibody (ab213676) at 1/300 dilution
Lane 1 : Mouse spleen tissue lysate
Lane 2 : Mouse thymus tissue lysate
Predicted band size: 89 kDaIncubated with the primary antibody overnight at 4°C.
Incubated with the secondary antibody for 60 minutes at 37°C.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR2 antibody (ab213676)Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat ovary tissue labeling TLR2 with ab213676 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
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Flow Cytometry - Anti-TLR2 antibody (ab213676)Flow cytometric analysis of TLR2 in Human A549 cells using ab213676 at 1/20 for 30 minutes, followed by a PE conjugated secondary antibody (green), compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).
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Western blot - Anti-TLR2 antibody (ab213676)Anti-TLR2 antibody (ab213676) at 1/1000 dilution + Cerebrum tissue lysate
Predicted band size: 89 kDaIncubated with the primary antibody overnight at 4°C.
Incubated with the secondary antibody for 60 minutes at 37°C.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR2 antibody (ab213676)Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat ovary tissue labeling TLR2 with ab213676 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
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Flow Cytometry - Anti-TLR2 antibody (ab213676)Flow cytometric analysis of TLR2 in mouse spleen cells using ab213676 at 1/20 for 30 minutes, followed by a PE conjugated secondary antibody (green), compared to control cells (blue), secondary only (light blue), and isotype control (orange).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR2 antibody (ab213676)Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat brain tissue labeling TLR2 with ab213676 at 1/400 dilution, followed by conjugation to the secondary antibody and DAB and counterstaining.
Antigen retrieval was performed by boiling in sodium citrate buffer (0.01M, pH6) for 15 minutes, blocked by endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, blocking buffer (normal goat serum) at 37°C for 20 minutes.
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Western blot - Anti-TLR2 antibody (ab213676)Anti-TLR2 antibody (ab213676) at 1/300 dilution + Raw264.7 cell lysate
Predicted band size: 89 kDaIncubated with the primary antibody overnight at 4°C.
Incubated with the secondary antibody for 60 minutes at 37°C.
