Anti-TLR2 antibody [EPR20303] (ab209217)
![Anti-TLR2 antibody [EPR20303] (ab209217)](https://www.abcam.com/ps/products/209/ab209217/Images/ab209217-272122-209217WB1.jpg "Anti-TLR2 antibody [EPR20303] (ab209217)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20303] to TLR2
- Suitable for: WB, IP
- Reacts with: Mouse, Rat
Overview
-
Product name
Anti-TLR2 antibody [EPR20303]
See all TLR2 primary antibodies -
Description
Rabbit monoclonal [EPR20303] to TLR2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP MouseWB Mouse
-
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: Mouse and rat spleen tissue lysates; RAW 264.7 whole cell lysates untreated and treated with 1 µg/ml lipopolysaccharides for 6 hours . IP: RAW 264.7 treated with 1 µg/ml LPS for 6 hours whole cell lysate.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20303 -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-TLR2 antibody [EPR20303] (ab209217)All lanes : Anti-TLR2 antibody [EPR20303] (ab209217) at 1/1000 dilution
Lane 1 : Mouse spleen tissue lysate
Lane 2 : Mouse brain tissue lysate
Lane 3 : Mouse heart tissue lysate
Lane 4 : Rat spleen tissue lysate
Lane 5 : Rat brain tissue lysate
Lane 6 : Rat heart tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 89 kDa
Observed band size: 89 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
TLR2 is expressed in spleen but not in brain and heart according to the literature (PMID: 11777946).
-
![Western blot - Anti-TLR2 antibody [EPR20303] (ab209217)](https://www.abcam.com/ps/products/209/ab209217/Images/ab209217-272121-anti-tlr2-antibody-epr20303-western-blot.jpg)
Western blot - Anti-TLR2 antibody [EPR20303] (ab209217)All lanes : Anti-TLR2 antibody [EPR20303] (ab209217) at 1/10000 dilution
Lane 1 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 2 : RAW 264.7 whole cell lysate treated with 1 µg/ml lipopolysaccharides for 6 hours
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 89 kDa
Observed band size: 89 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
Expression of TLR2 in RAW 264.7 cells can be enhanced by LPS treatment according to the literature (PMID: 11067935).
-
![Immunoprecipitation - Anti-TLR2 antibody [EPR20303] (ab209217)](https://www.abcam.com/ps/products/209/ab209217/Images/ab209217-272119-anti-tlr2-antibody-epr20303-immunoprecipitation.jpg)
Immunoprecipitation - Anti-TLR2 antibody [EPR20303] (ab209217)TLR2 was immunoprecipitated from 0.35 mg of RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate, treated with 1 µg/ml LPS for 6 hours, with ab209217 at 1/30 dilution.
Western blot was performed from the immunoprecipitate using ab209217 at 1/500 dilution.
VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Lane 1: RAW 264.7 whole cell lysate treated with 1 µg/ml LPS for 6 hours, 10 µg (Input).
Lane 2: ab209217 IP in RAW 264.7 whole cell lysate treated with 1 µg/ml LPS for 6 hours.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab209217 in RAW 264.7 whole cell lysate treated with 1 µg/ml LPS for 6 hours.Blocking and dilution buffer and concentration: 5% NFDM/TBST.
-
![Anti-TLR2 antibody [EPR20303] (ab209217)](https://www.abcam.com/ps/products/209/ab209217/Images/ab209217-5-benefits-of-recombinant-antibodies.png)
Anti-TLR2 antibody [EPR20303] (ab209217)
