Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PCK2 antibody [EPR14223(B)] (ab181170)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14223(B)] to PCK2
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-PCK2 antibody [EPR14223(B)]
    See all PCK2 primary antibodies

  • Description

    Rabbit monoclonal [EPR14223(B)] to PCK2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Mouse
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HepG2, MCF7 or A431 lysate, Human liver or Mouse kidney tissue.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCK2 antibody [EPR14223(B)] (ab181170)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCK2 antibody [EPR14223(B)] (ab181170)

    Immunohistochemical analysis of paraffin embedded Mouse kidney tissue labeling PCK2 with ab181170 at a 1/250 dilution. Prediluted HRP Polymer for Rabbit IgG secondary used. Counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCK2 antibody [EPR14223(B)] (ab181170)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCK2 antibody [EPR14223(B)] (ab181170)

    Immunohistochemical analysis of paraffin embedded Human liver tissue labeling PCK2 with ab181170 at a 1/250 dilution. Prediluted HRP Polymer for Rabbit IgG secondary used. Counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Western blot - Anti-PCK2 antibody [EPR14223(B)] (ab181170)
    Western blot - Anti-PCK2 antibody [EPR14223(B)] (ab181170)
    All lanes : Anti-PCK2 antibody [EPR14223(B)] (ab181170) at 1/1000 dilution

    Lane 1 : MCF7 lysate
    Lane 2 : A431 lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 71 kDa

  • Western blot - Anti-PCK2 antibody [EPR14223(B)] (ab181170)
    Western blot - Anti-PCK2 antibody [EPR14223(B)] (ab181170)
    Anti-PCK2 antibody [EPR14223(B)] (ab181170) at 1/10000 dilution + HepG2 lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 71 kDa

  • Anti-PCK2 antibody [EPR14223(B)] (ab181170)
    Anti-PCK2 antibody [EPR14223(B)] (ab181170)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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