Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PCK2 antibody [EPR14224] - C-terminal (ab187145)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14224] to PCK2 - C-terminal
  • Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PCK2 antibody [EPR14224] - C-terminal
    See all PCK2 primary antibodies

  • Description

    Rabbit monoclonal [EPR14224] to PCK2 - C-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HepG2, MCF7, HeLa, mouse brain, mouse kidney, rat brain, rat kidney and rat spleen lysates; Human liver tissue; HeLa cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-PCK2 antibody [EPR14224] - C-terminal (ab187145)
    Western blot - Anti-PCK2 antibody [EPR14224] - C-terminal (ab187145)
    Anti-PCK2 antibody [EPR14224] - C-terminal (ab187145) at 1/10000 dilution + HepG2 lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 71 kDa

  • Western blot - Anti-PCK2 antibody [EPR14224] - C-terminal (ab187145)
    Western blot - Anti-PCK2 antibody [EPR14224] - C-terminal (ab187145)
    All lanes : Anti-PCK2 antibody [EPR14224] - C-terminal (ab187145) at 1/1000 dilution

    Lane 1 : MCF7 lysate
    Lane 2 : Hela lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 71 kDa

  • Western blot - Anti-PCK2 antibody [EPR14224] - C-terminal (ab187145)
    Western blot - Anti-PCK2 antibody [EPR14224] - C-terminal (ab187145)
    All lanes : Anti-PCK2 antibody [EPR14224] - C-terminal (ab187145) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Mouse kidney lysate
    Lane 3 : Mouse spleen lysate
    Lane 4 : Rat brain lysate
    Lane 5 : Rat kidney lysate
    Lane 6 : Rat spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 71 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-PCK2 antibody [EPR14224] - C-terminal (ab187145)
    Immunocytochemistry/ Immunofluorescence - Anti-PCK2 antibody [EPR14224] - C-terminal (ab187145)

    Immunofluorescence analysis of 4% paraformaldehyde-fixed HeLa cells, labeling PCK2 (green) with ab187145 at 1/500 dilution. Alexa Fluor®488-conjugated goat anti-rabbit IgG was used as a secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue).

  • Flow Cytometry - Anti-PCK2 antibody [EPR14224] - C-terminal (ab187145)
    Flow Cytometry - Anti-PCK2 antibody [EPR14224] - C-terminal (ab187145)

    Flow cytometry analysis of PCK2 expression in 2% paraformaldehyde-fixed HeLa cells using ab187145 at 1/50 dilution (red) and a rabbit IgG as negative control (green). A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCK2 antibody [EPR14224] - C-terminal (ab187145)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCK2 antibody [EPR14224] - C-terminal (ab187145)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue, labeling PCK2 with ab187145 at 1/500 dilution. Detected using HRP Polymer for Rabbit IgG and counter-stained using hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Anti-PCK2 antibody [EPR14224] - C-terminal (ab187145)
    Anti-PCK2 antibody [EPR14224] - C-terminal (ab187145)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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