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Immunology Innate Immunity Macrophage / Inflamm.

Anti-PF4 antibody [EPR17279-1] (ab182988)

Price and availability

278 083 ₸

Availability

Order now and get it on Wednesday March 17, 2021

Anti-PF4 antibody [EPR17279-1] (ab182988)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17279-1] to PF4
  • Suitable for: WB, IP
  • Reacts with: Mouse

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Overview

  • Product name

    Anti-PF4 antibody [EPR17279-1]
    See all PF4 primary antibodies
  • Description

    Rabbit monoclonal [EPR17279-1] to PF4
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IP
    Mouse
    WB
    Mouse
    See all applications and species data
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse serum and platelet lysate. IP: Mouse serum.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR17279-1
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Cardiovascular
    • Blood
    • Platelets
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Leukocyte recruitment
    • Chemokines

Images

  • Western blot - Anti-PF4 antibody [EPR17279-1] (ab182988)
    Western blot - Anti-PF4 antibody [EPR17279-1] (ab182988)
    All lanes : Anti-PF4 antibody [EPR17279-1] (ab182988) at 1/1000 dilution

    Lane 1 : Mouse serum lysate
    Lane 2 : Mouse platelet lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 11 kDa
    Observed band size: 11 kDa



    Blocking buffer: 5% NFDM/TBST.

    Exposure times:

    Lane 1: 3 minutes;

    Lane 2: 1 second.

  • Immunoprecipitation - Anti-PF4 antibody [EPR17279-1] (ab182988)
    Immunoprecipitation - Anti-PF4 antibody [EPR17279-1] (ab182988)

    PF4 was immunoprecipitated from 0.35 mg mouse serum lysate with ab182988 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab182988 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) used at 1/10,000 dilution.
    Lane 1: Mouse serum lysate 10 µg (Input).
    Lane 2: ab182988 IP in mouse serum lysate (+).
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab182988 in mouse serum (-).
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 1 second.

  • Anti-PF4 antibody [EPR17279-1] (ab182988)
    Anti-PF4 antibody [EPR17279-1] (ab182988)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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