Anti-PF4 antibody (ab9561)
Key features and details
- Rabbit polyclonal to PF4
- Suitable for: WB, Sandwich ELISA
- Reacts with: Human
- Isotype: unknown
Overview
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Product name
Anti-PF4 antibody
See all PF4 primary antibodies -
Description
Rabbit polyclonal to PF4 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species sELISA HumanWB Human
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Immunogen
Highly pure (>98%) recombinant hPF-4 (human Platelet Factor-4)
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Positive control
- Recombinant human PF4 protein (ab9562) can be used as a positive control in WB.
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General notes
This product is no longer batch tested in IHC, for an IHC validated antibody please see ab49735
Properties
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Form
Lyophilized:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary. -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
unknown -
Light chain type
unknown -
Research areas
Images
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Western blot - Anti-PF4 antibody (ab9561)Anti-PF4 antibody (ab9561) at 1 µg/ml + Human Platelet (Human adult normal cell line) Whole Cell Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 11 kDa
Observed band size: 11 kDa
Additional bands at: 18 kDa, 30 kDa, 37 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes
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Sandwich ELISA - Anti-PF4 antibody (ab9561)To detect Human PF-4 by sandwich ELISA (using 100μl/well) a concentration of 0.5-2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with suitable detection antibody, allows the detection of at least 2000-4000 pg/ml of Recombinant Human PF-4.
