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Anti-PF4 antibody [EPR17279-1] - BSA and Azide free (ab227485)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17279-1] to PF4 - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Mouse

Overview

  • Product name

    Anti-PF4 antibody [EPR17279-1] - BSA and Azide free
    See all PF4 primary antibodies

  • Description

    Rabbit monoclonal [EPR17279-1] to PF4 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Mouse
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse serum and platelet lysate.

  • General notes

    Ab227485 is the carrier-free version of ab182988. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab227485 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunoprecipitation - Anti-PF4 antibody [EPR17279-1] - BSA and Azide free (ab227485)
    Immunoprecipitation - Anti-PF4 antibody [EPR17279-1] - BSA and Azide free (ab227485)

    PF4 was immunoprecipitated from 0.35 mg mouse serum lysate with ab182988 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab182988 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/10,000 dilution.
    Lane 1: Mouse serum lysate 10 µg (Input).
    Lane 2: ab182988 IP in mouse serum lysate (+).
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab182988 in mouse serum (-).
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 1 second.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab182988).

  • Western blot - Anti-PF4 antibody [EPR17279-1] - BSA and Azide free (ab227485)
    Western blot - Anti-PF4 antibody [EPR17279-1] - BSA and Azide free (ab227485)
    All lanes : Anti-PF4 antibody [EPR17279-1] (ab182988) at 1/1000 dilution

    Lane 1 : Mouse serum lysate
    Lane 2 : Mouse platelet lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 11 kDa
    Observed band size: 11 kDa



    Blocking buffer: 5% NFDM/TBST.

    Exposure times:

    Lane 1: 3 minutes;

    Lane 2: 1 second.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab182988).

  • Anti-PF4 antibody [EPR17279-1] - BSA and Azide free (ab227485)
    Anti-PF4 antibody [EPR17279-1] - BSA and Azide free (ab227485)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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