Myeloperoxidase (MPO) Activity Assay Kit (Fluorometric) (ab111749)
Key features and details
- Assay type: Enzyme activity
- Detection method: Fluorescent
- Platform: Microplate reader
- Assay time: 30 min
- Sample type: Cell culture supernatant, Tissue Extracts
- Sensitivity: 0.0005 mU/well
Overview
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Product name
Myeloperoxidase (MPO) Activity Assay Kit (Fluorometric)
See all Myeloperoxidase kits -
Detection method
Fluorescent -
Sample type
Cell culture supernatant, Tissue Extracts -
Assay type
Enzyme activity -
Sensitivity
> 0.0005 mU/well -
Assay time
0h 30m -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Myeloperoxidase (MPO) Activity Assay Kit ab111749 provides a rapid, simple and reliable fluorometric assay to study MPO activity.
In the MPO assay protocol, MPO catalyzes the production of sodium hypochlorite (NaClO) from hydrogen peroxide (H2O2) and sodium chloride (NaCl). Subsequently, NaClO reacts stoichiometrically with the free radical sensor Aminophenyl fluorescein (APF) to generate fluorescein, whose fluorescence can be detected at Ex/Em = 485/525 nm.
This MPO assay kit can be used to detect MPO activity as low as 0.5 µU per well.
MPO assay protocol summary:
- add fluoroscein standard to wells and analyze with a microplate reader
- add samples to wells
- add reaction mix
- analyze every 2-3 mins with a microplate reader in kinetic mode for at least 30 mins -
Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests Assay Buffer WM 1 x 25ml Fluorescein Standard (1 mM) Yellow cap 1 x 50µl MPO Positive Control Purple cap 1 vial MPO Probe Red cap 1 x 200µl MPO Substrate Stock Blue cap 1 x 50µl -
Research areas
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Function
Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity. -
Involvement in disease
Defects in MPO are the cause of myeloperoxidase deficiency (MPD) [MIM:254600]. MPD is an autosomal recessive defect that results in disseminated candidiasis. -
Sequence similarities
Belongs to the peroxidase family. XPO subfamily. -
Cellular localization
Lysosome. - Information by UniProt
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Alternative names
- 84 kDa myeloperoxidase
- 89 kDa myeloperoxidase
- EC 1.11.1.7
see all
Images
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MPO activity - ab111749
a. Measurement of MPO activity in WBC lysate (0.1 μg) and MPO Positive Control (3 μL). b. MPO specific activity in WBC lysate.
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Functional Studies - Myeloperoxidase (MPO) Fluorometric Activity Assay Kit (ab111749)Standard Curve using this kit protocol (Ex/Em = 485/525 nm)
