Myeloperoxidase (MPO) Activity Assay Kit (Colorimetric) (ab105136)
Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 1 hr
- Sample type: Cell culture supernatant, Other biological fluids, Serum, Tissue Homogenate, Urine
Overview
-
Product name
Myeloperoxidase (MPO) Activity Assay Kit (Colorimetric)
See all Myeloperoxidase kits -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Urine, Serum, Other biological fluids, Tissue Homogenate -
Assay type
Enzyme activity -
Assay time
1h 00m -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Myeloperoxidase (MPO) Activity Assay Kit ab105136 is a rapid, simple, sensitive, and reliable colorimetric assay suitable for use as a high throughput MPO activity assay.
In the MPO assay protocol, myeloperoxidase produces HClO from H2O2 and Cl-. The HClO reacts with taurine to generate the taurine chloramine, which subsequently reacts with the DTNB probe to eliminate color (absorbance at 412 nm). The absorbance is inversely proportional to the amount of MPO enzyme.
This MPO assay kit can be used to detect myeloperoxidase as low as 0.05 mU per well.
MPO assay protocol summary:
- add samples to wells (and assay buffer only to wells for standards)
- add reaction mix to sample wells and incubate for 30 min to 2 hrs
- add stop mix and incubate for 10 min
- add TNB reagent/standard to all wells and incubate for 5-10 min
- analyze with a microplate reader -
Notes
Myeloperoxidase (MPO) is a peroxidase enzyme (EC 1.11.1.7) most abundantly expressed in neutrophil granulocytes. It is a lysosomal protein stored in the azurophilic granules of the neutrophil. Myeloperoxidase contains a heme pigment which causes its green color in secretions rich in neutrophils, such as pus and some forms of mucus. Myeloperoxidase catalyzes the production of hypochlorous acid (HClO) from hydrogen peroxide (H2O2) and chloride anion (Cl-, or the equivalent from a non-chlorine halide). Myeloperoxidase also oxidizes tyrosine to a tyrosyl radical using hydrogen peroxide as an oxidizing agent.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate reader
Properties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests DTNB Probe Red 1 x 50µl MPO Assay Buffer WM 1 x 25ml MPO Positive Control (lyophilized) Purple 1 vial MPO Substrate Stock Blue 1 x 50µl Stop Mix (lyophilized) Green 1 x 20µl TCEP (50mM) Clear 1 x 50µl -
Research areas
-
Function
Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity. -
Involvement in disease
Defects in MPO are the cause of myeloperoxidase deficiency (MPD) [MIM:254600]. MPD is an autosomal recessive defect that results in disseminated candidiasis. -
Sequence similarities
Belongs to the peroxidase family. XPO subfamily. -
Cellular localization
Lysosome. - Information by UniProt
-
Alternative names
- 84 kDa myeloperoxidase
- 89 kDa myeloperoxidase
- EC 1.11.1.7
see all
Images
-
Functional Studies - Myeloperoxidase (MPO) Activity Assay Kit (Colorimetric) (ab105136) Namkoong, Ho et al., PLoS pathogens?vol. 14,4 e1006955., S4 fig, doi:10.1371/journal.ppat.1006955MPO activity in indicated cells was measured using the commercially available MPO Activity Assay Kit (n = 3).
-
MPO activitya. Measurement of MPO activity using WBC lysate (3 μg) and MPO Positive Control (5 and 10 μL). b. MPO specific activity in WBC lysate.
-
Typical Standard CurveTypical standard curve for ab105136.
