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Immunology Innate Immunity Macrophage / Inflamm.

Anti-CD32A + CD32C antibody [EPR6658] - BSA and Azide free (ab248099)

Price and availability

526 012 ₸

Availability

Order now and get it on Tuesday March 09, 2021

Anti-CD32A + CD32C antibody [EPR6658] - BSA and Azide free (ab248099)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6658] to CD32A + CD32C - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Human

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Overview

  • Product name

    Anti-CD32A + CD32C antibody [EPR6658] - BSA and Azide free
  • Description

    Rabbit monoclonal [EPR6658] to CD32A + CD32C - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: Flow Cyt,ICC,ICC/IF or IP
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab248099 is the carrier-free version of ab125013. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab248099 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Dissociation constant (KD)

    KD = 8.90 x 10 -10 M
    Learn more about KD
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR6658
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Immunology
    • Immunoglobulins
    • Receptors
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • B Lymphocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • T Lymphocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Monocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Neutrophil Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Mast Cell Lineage

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32A + CD32C antibody [EPR6658] - BSA and Azide free (ab248099)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32A + CD32C antibody [EPR6658] - BSA and Azide free (ab248099)
    This data was developed using ab125013, the same antibody clone in a different buffer formulation.ab125013 at 1/250 staining CD32A + CD32C in paraffin-embedded Human lung tissue by immunohistochemistry. Heat mediated antigen retrieval was perrformed before commencing with IHC staining protocol.
  • Western blot - Anti-CD32A + CD32C antibody [EPR6658] - BSA and Azide free (ab248099)
    Western blot - Anti-CD32A + CD32C antibody [EPR6658] - BSA and Azide free (ab248099)
    All lanes : Anti-CD32A + CD32C antibody [EPR6658] (ab125013) at 1/1000 dilution

    Lane 1 : K562 lysate
    Lane 2 : Human platelet lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 35 kDa
    Observed band size: 37-43 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab125013, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32A + CD32C antibody [EPR6658] - BSA and Azide free (ab248099)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32A + CD32C antibody [EPR6658] - BSA and Azide free (ab248099)
    This data was developed using ab125013, the same antibody clone in a different buffer formulation.ab125013 at 1/250 staining CD32A + CD32C in paraffin-embedded Human spleen tissue by immunohistochemistry. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
  • OI-RD Scanning - Anti-CD32A + CD32C antibody [EPR6658] - BSA and Azide free (ab248099)
    OI-RD Scanning - Anti-CD32A + CD32C antibody [EPR6658] - BSA and Azide free (ab248099)
    This data was developed using ab125013, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-CD32A + CD32C antibody [EPR6658] - BSA and Azide free (ab248099)
    Anti-CD32A + CD32C antibody [EPR6658] - BSA and Azide free (ab248099)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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