Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-EAAT2 antibody [EPR19798] - BSA and Azide free (ab271970)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19798] to EAAT2 - BSA and Azide free
  • Suitable for: IP, IHC-P, WB, IHC-Fr
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-EAAT2 antibody [EPR19798] - BSA and Azide free
    See all EAAT2 primary antibodies

  • Description

    Rabbit monoclonal [EPR19798] to EAAT2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, IHC-P, WB, IHC-Frmore details

  • Species reactivity

    Reacts with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Mouse and rat striatum tissues. IHC-Fr: Mouse striatum tissue. IP: Mouse brain lysate.

  • General notes

    ab271970 is the carrier-free version of ab205248. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR19798

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT2 antibody [EPR19798] - BSA and Azide free (ab271970)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT2 antibody [EPR19798] - BSA and Azide free (ab271970)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling EAAT2 with ab205248 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Negative control: no staining on mouse kidney [PMID: 11038258].

    Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab205248).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT2 antibody [EPR19798] - BSA and Azide free (ab271970)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT2 antibody [EPR19798] - BSA and Azide free (ab271970)

    Immunohistochemical analysis of paraffin-embedded mouse striatum tissue labeling EAAT2 with ab205248 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Membrane staining on mouse striatum is observed [PMID 25391854]. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab205248).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT2 antibody [EPR19798] - BSA and Azide free (ab271970)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT2 antibody [EPR19798] - BSA and Azide free (ab271970)

    Immunohistochemical analysis of paraffin-embedded rat striatum tissue labeling EAAT2 with ab205248 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Membrane staining on Rat striatum is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab205248).
  • Immunohistochemistry (Frozen sections) - Anti-EAAT2 antibody [EPR19798] - BSA and Azide free (ab271970)
    Immunohistochemistry (Frozen sections) - Anti-EAAT2 antibody [EPR19798] - BSA and Azide free (ab271970)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Mouse striatum tissue labeling EAAT2 with ab205248 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Membrane staining on mouse striatum is observed [PMID: 25391854].  The nuclear counterstain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077  at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab205248).
  • Immunoprecipitation - Anti-EAAT2 antibody [EPR19798] - BSA and Azide free (ab271970)
    Immunoprecipitation - Anti-EAAT2 antibody [EPR19798] - BSA and Azide free (ab271970)

    EAAT2 was immunoprecipitated from 0.35 mg of Mouse brain lysate with ab205248 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab205248 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: Mouse brain lysate, 10μg (Input).

    Lane 2: ab205248 IP in Mouse brain lysate.

    Lane 3: Rabbit IgG,monoclonal [EPR25A]- Isotype Control (ab172730) instead of ab205248 in Mouse brain lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab205248).
  • Anti-EAAT2 antibody [EPR19798] - BSA and Azide free (ab271970)
    Anti-EAAT2 antibody [EPR19798] - BSA and Azide free (ab271970)

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