Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

Order now and get it on Tuesday March 09, 2021

Anti-EAAT2 antibody [EPR19794] (ab205247)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR19794] to EAAT2
Suitable for: WB, IHC-P, IP
Reacts with: Mouse, Rat

Product name

Anti-EAAT2 antibody [EPR19794]
See all EAAT2 primary antibodies
Description

Rabbit monoclonal [EPR19794] to EAAT2
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Mouse
IP	Rat
WB	Mouse Rat

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Mouse and rat brain lysates. IHC-P: Mouse hippocampus and liver tissues. IP: Rat brain lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR19794
Isotype

IgG
Research areas

Western blot - Anti-EAAT2 antibody [EPR19794] (ab205247)

All lanes : Anti-EAAT2 antibody [EPR19794] (ab205247) at 1/2000 dilution

Lane 1 : Mouse brain lysate
Lane 2 : Rat brain lysate
Lane 3 : Mouse liver lysate
Lane 4 : Mouse spleen lysate
Lane 5 : Rat liver lysate
Lane 6 : Rat kidney lysate
Lane 7 : Rat spleen lysate
Lane 8 : C6 (Rat glial tumor cell line) whole cell lysate
Lane 9 : Neuro-2a (Mouse neuroblastoma cell line) whole cell lysate
Lane 10 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 11 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 12 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 62 kDa
Observed band size: 180,65 kDa
why is the actual band size different from the predicted?

Exposure time: 5 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.

EAAT2 is a multi-pass membrane protein, the main transporter that clears the excitatory neurotransmitter glutamate in the central nervous system. The bands around 180KD are multimers of EAAT2, which is consistent with what have been described in the literatures (PMID: 24569372 and 20193040).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT2 antibody [EPR19794] (ab205247)

Immunohistochemical analysis of paraffin-embedded Mouse hippocampus tissue labeling EAAT2 with ab205247 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Membrane staining on neurons of mouse hippocampus is observed [PMID: 25391854].

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT2 antibody [EPR19794] (ab205247)

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling EAAT2 with ab205247 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Membrane staining on neurons of mouse liver is observed [PMID: 19718708].

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT2 antibody [EPR19794] (ab205247)

Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling EAAT2 with ab205247 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Negative staining on mouse kidney.

Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunoprecipitation - Anti-EAAT2 antibody [EPR19794] (ab205247)

EAAT2 was immunoprecipitated from 0.35 mg of Rat brain lysate with ab205247 at 1/40 dilution.

Western blot was performed from the immunoprecipitate using ab205247 at 1/2000 dilution.

VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1: Rat brain lysate, 10µg (Input).

Lane 2: ab205247 IP in Rat brain lysate.

Lane 3: Rabbit IgG,monoclonal [EPR25A]-Isotype Control (ab172730) instead of ab205247 in Rat brain lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 1 second.
Anti-EAAT2 antibody [EPR19794] (ab205247)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com