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Neuroscience Neurotransmitter Transporters Glutamate

Anti-Glutamate receptor 3/GluA3 antibody [EP813Y] (ab40845)

Price and availability

298 185 ₸

Availability

Order now and get it on Thursday March 04, 2021

Anti-Glutamate receptor 3/GluA3 antibody [EP813Y] (ab40845)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP813Y] to Glutamate receptor 3/GluA3
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-Glutamate receptor 3/GluA3 antibody [EP813Y]
    See all Glutamate receptor 3/GluA3 primary antibodies
  • Description

    Rabbit monoclonal [EP813Y] to Glutamate receptor 3/GluA3
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Mouse
    Rat
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human Glutamate receptor 3/GluA3 aa 250-350. The exact sequence is proprietary.
    Database link: P42263

  • Positive control

    • WB: Human brain tissue lysate.
  • General notes

    Previously labelled as Glutamate receptor 3. 

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 0.05% BSA, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP813Y
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmitter
    • Amino Acids
    • Glutamate
    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • GPCR
    • Glutamate Receptors
    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • Ligand-Gated Ion Channels
    • AMPA / Kainate
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • GPCR

Images

  • Western blot - Anti-Glutamate receptor 3/GluA3 antibody [EP813Y] (ab40845)
    Western blot - Anti-Glutamate receptor 3/GluA3 antibody [EP813Y] (ab40845)
    Anti-Glutamate receptor 3/GluA3 antibody [EP813Y] (ab40845) at 1/1000 dilution (purified) + Human hippocampus lysates at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 99 kDa



    Blocking and diluting buffer: 5% NFDM/TBST

  • Western blot - Anti-Glutamate receptor 3/GluA3 antibody [EP813Y] (ab40845)
    Western blot - Anti-Glutamate receptor 3/GluA3 antibody [EP813Y] (ab40845)
    All lanes : Anti-Glutamate receptor 3/GluA3 antibody [EP813Y] (ab40845) at 1/2000 dilution (purified)

    Lane 1 : Mouse hippocampus lysates
    Lane 2 : Rat brain lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

    Predicted band size: 99 kDa
    Observed band size: 100 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer: 5% NFDM/TBST

  • Western blot - Anti-Glutamate receptor 3/GluA3 antibody [EP813Y] (ab40845)
    Western blot - Anti-Glutamate receptor 3/GluA3 antibody [EP813Y] (ab40845)
    Anti-Glutamate receptor 3/GluA3 antibody [EP813Y] (ab40845) at 1/5000 dilution + human brain lysate at 10 µg

    Predicted band size: 99 kDa
    Observed band size: 95 kDa why is the actual band size different from the predicted?

  • Anti-Glutamate receptor 3/GluA3 antibody [EP813Y] (ab40845)
    Anti-Glutamate receptor 3/GluA3 antibody [EP813Y] (ab40845)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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