Anti-EAAT2 antibody (ab235202)
Key features and details
- Goat polyclonal to EAAT2
- Suitable for: WB, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
Overview
-
Product name
Anti-EAAT2 antibody
See all EAAT2 primary antibodies -
Description
Goat polyclonal to EAAT2 -
Host species
Goat -
Specificity
Recommended for rat samples only.
-
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF RatWB RatHuman
-
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- ICC/IF: Rat pup brain heterogenous cells. WB: Rat brain lysate.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-EAAT2 antibody (ab235202)All lanes : Anti-EAAT2 antibody (ab235202) at 5 µg/ml
Lane 1 : Rat brain lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lysates/proteins at 35 µg per lane.
Secondary
All lanes : Goat IgG [H&L] HRP at 1/40000 dilution
Predicted band size: 62 kDa
Exposure time: 60 secondsPredicted MW: 60, 62 kDa (glycosylations and lipidations).
Dimerization expected.
-
Immunocytochemistry/ Immunofluorescence - Anti-EAAT2 antibody (ab235202)4% paraformaldehyde-fixed, 0.3% Triton X-100 permeabilized rat pup brain heterogenous cells stained for EAAT2 (green) using ab235202 at 15 μg/ml (overnight at 4°C) in ICC/IF. Donkey Anti-Goat IgG DyLight™ 488 Preadsorbed was used as the secondary antibody 5 µg/ml (1 hour at RT). Nuclear counterstain was DAPI.
