Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-EAAT2 antibody [EPR19794] - BSA and Azide free (ab251423)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19794] to EAAT2 - BSA and Azide free
  • Suitable for: WB, IHC-P, IP
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-EAAT2 antibody [EPR19794] - BSA and Azide free
    See all EAAT2 primary antibodies

  • Description

    Rabbit monoclonal [EPR19794] to EAAT2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251423 is the carrier-free version of ab205247. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251423 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Clonality

    Monoclonal

  • Clone number

    EPR19794

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-EAAT2 antibody [EPR19794] - BSA and Azide free (ab251423)
    Western blot - Anti-EAAT2 antibody [EPR19794] - BSA and Azide free (ab251423)
    All lanes : Anti-EAAT2 antibody [EPR19794] (ab205247) at 1/2000 dilution

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Rat brain tissue lysate
    Lane 3 : Mouse liver tissue lysate
    Lane 4 : Mouse spleen tissue lysate
    Lane 5 : Rat liver tissue lysate
    Lane 6 : Rat kidney tissue lysate
    Lane 7 : Rat spleen tissue lysate
    Lane 8 : C6 (Rat glial tumor cell line) whole cell lysate
    Lane 9 : Neuro-2a (Mouse neuroblastoma cell line) whole cell lysate
    Lane 10 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 11 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
    Lane 12 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 62 kDa
    Observed band size: 180,65 kDa
    why is the actual band size different from the predicted?


    Exposure time: 5 seconds


    This data was developed using ab205247, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    EAAT2 is a multi-pass membrane protein, the main transporter that clears the excitatory neurotransmitter glutamate in the central nervous system. The bands around 180KD are multimers of EAAT2, which is consistent with what have been described in the literatures (PMID: 24569372 and 20193040).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT2 antibody [EPR19794] - BSA and Azide free (ab251423)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT2 antibody [EPR19794] - BSA and Azide free (ab251423)
    This data was developed using ab205247, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Mouse hippocampus tissue labeling EAAT2 with ab205247 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Membrane staining on neurons of mouse hippocampus is observed [PMID: 25391854]. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT2 antibody [EPR19794] - BSA and Azide free (ab251423)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT2 antibody [EPR19794] - BSA and Azide free (ab251423)
    This data was developed using ab205247, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling EAAT2 with ab205247 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Membrane staining on neurons of mouse liver is observed [PMID: 19718708]. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT2 antibody [EPR19794] - BSA and Azide free (ab251423)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT2 antibody [EPR19794] - BSA and Azide free (ab251423)
    This data was developed using ab205247, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling EAAT2 with ab205247 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative staining on mouse kidney. Counter stained with Hematoxylin. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunoprecipitation - Anti-EAAT2 antibody [EPR19794] - BSA and Azide free (ab251423)
    Immunoprecipitation - Anti-EAAT2 antibody [EPR19794] - BSA and Azide free (ab251423)
    This data was developed using ab205247, the same antibody clone in a different buffer formulation.EAAT2 was immunoprecipitated from 0.35 mg of Rat brain lysate with ab205247 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab205247 at 1/2000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution. Lane 1: Rat brain lysate, 10µg (Input). Lane 2: ab205247 IP in Rat brain lysate. Lane 3: Rabbit IgG,monoclonal [EPR25A]-Isotype Control (ab172730) instead of ab205247 in Rat brain lysate. Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 1 second.
  • Anti-EAAT2 antibody [EPR19794] - BSA and Azide free (ab251423)
    Anti-EAAT2 antibody [EPR19794] - BSA and Azide free (ab251423)

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