Anti-Caveolin-3 antibody [EPR18975] - BSA and Azide free (ab250637)
![Anti-Caveolin-3 antibody [EPR18975] - BSA and Azide free (ab250637)](https://www.abcam.com/ps/products/250/ab250637/Images/ab250637-446459-anti-caveolin-3-antibody-epr18975-bsa-and-azide-free-western-blot.jpg "Anti-Caveolin-3 antibody [EPR18975] - BSA and Azide free (ab250637)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18975] to Caveolin-3 - BSA and Azide free
- Suitable for: IP, IHC-P, WB
- Reacts with: Human
Overview
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Product name
Anti-Caveolin-3 antibody [EPR18975] - BSA and Azide free
See all Caveolin-3 primary antibodies -
Description
Rabbit monoclonal [EPR18975] to Caveolin-3 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IP, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab250637 is the carrier-free version of ab182739.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18975 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Caveolin-3 antibody [EPR18975] - BSA and Azide free (ab250637)All lanes : Anti-Caveolin-3 antibody [EPR18975] (ab182739) at 1/1000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : Human fetal heart lysate
Lane 3 : Human fetal kidney lysate
Lane 4 : Human fetal spleen lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 17 kDa
Observed band size: 17 kDa
Exposure time: 5 secondsThis data was developed using ab182739, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Expressed predominantly in muscle.
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![Western blot - Anti-Caveolin-3 antibody [EPR18975] - BSA and Azide free (ab250637)](https://www.abcam.com/ps/products/250/ab250637/Images/ab250637-445640-anti-caveolin-3-antibody-epr18975-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-Caveolin-3 antibody [EPR18975] - BSA and Azide free (ab250637)All lanes : Anti-Caveolin-3 antibody [EPR18975] (ab182739) at 1/1000 dilution
Lane 1 : Human skeletal muscle lysate
Lane 2 : Human fetal lung lysate
Lane 3 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
Lane 4 : A-673 (Human muscle Ewing's Sarcoma cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 17 kDa
Observed band size: 17 kDa
Exposure time: 30 secondsThis data was developed using ab182739, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Expressed predominantly in muscle.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody [EPR18975] - BSA and Azide free (ab250637)](https://www.abcam.com/ps/products/250/ab250637/Images/ab250637-5-anti-caveolin-3-antibody-epr18975-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody [EPR18975] - BSA and Azide free (ab250637)This data was developed using ab182739, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling Caveolin-3 with ab182739 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Membrane staining on Human skeletal muscle was observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody [EPR18975] - BSA and Azide free (ab250637)](https://www.abcam.com/ps/products/250/ab250637/Images/ab250637-4-anti-caveolin-3-antibody-epr18975-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody [EPR18975] - BSA and Azide free (ab250637)This data was developed using ab182739, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling Caveolin-3 with ab182739 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Membrane staining on Human cardiac muscle was observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody [EPR18975] - BSA and Azide free (ab250637)](https://www.abcam.com/ps/products/250/ab250637/Images/ab250637-3-anti-caveolin-3-antibody-epr18975-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody [EPR18975] - BSA and Azide free (ab250637)This data was developed using ab182739, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Caveolin-3 with ab182739 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative on Human liver. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody [EPR18975] - BSA and Azide free (ab250637)](https://www.abcam.com/ps/products/250/ab250637/Images/ab250637-2-anti-caveolin-3-antibody-epr18975-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody [EPR18975] - BSA and Azide free (ab250637)This data was developed using ab182739, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling Caveolin-3 with ab182739 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative on Human hepatocellular carcinoma. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. -
![Immunoprecipitation - Anti-Caveolin-3 antibody [EPR18975] - BSA and Azide free (ab250637)](https://www.abcam.com/ps/products/250/ab250637/Images/ab250637-399634-anti-caveolin-3-antibody-epr18975-bsa-and-azide-free-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Caveolin-3 antibody [EPR18975] - BSA and Azide free (ab250637)This data was developed using ab182739, the same antibody clone in a different buffer formulation.
Caveolin-3 was immunoprecipitated from 1mg of Human fetal heart whole cell lysate with ab182739 at 1/20 dilution. Western blot was performed from the immunoprecipitate using ab182739 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: Human fetal heart whole cell lysate 10ug (Input).
Lane 2: ab182739 IP in Human fetal heart whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab182739 in Human fetal heart whole cell lysate.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 10 seconds.
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![Anti-Caveolin-3 antibody [EPR18975] - BSA and Azide free (ab250637)](https://www.abcam.com/ps/products/250/ab250637/Images/ab250637-6-benefits-of-recombinant-antibodies.png)
Anti-Caveolin-3 antibody [EPR18975] - BSA and Azide free (ab250637)