Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MEF2C antibody [EPR19089-202] - BSA and Azide free (ab231859)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19089-202] to MEF2C - BSA and Azide free
  • Suitable for: WB, ICC/IF, ChIP, Flow Cyt, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-MEF2C antibody [EPR19089-202] - BSA and Azide free
    See all MEF2C primary antibodies

  • Description

    Rabbit monoclonal [EPR19089-202] to MEF2C - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ChIP
    Human
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human skeletal muscle tissue.

  • General notes

    Ab231859 is the carrier-free version of ab211493. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab231859 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Flow Cytometry - Anti-MEF2C antibody [EPR19089-202] - BSA and Azide free (ab231859)
    Flow Cytometry - Anti-MEF2C antibody [EPR19089-202] - BSA and Azide free (ab231859)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilsed Jurkat (Human T cell leukemia T lymphocyte, Left) / Raji (Human Burkitt's lymphoma B lymphocyte, Right) cell lines labelling MEF2C with ab211493 at 1/500 dilution (Red) compared with the isotype control Rabbit monoclonal IgG (ab172730) (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG Alexa Fluor® 488 (ab150077), at 1/2000 dilution was used as the secondary antibody.

    Negative control: Jurkat (PMID: 27876533).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab211493).

  • ChIP - Anti-MEF2C antibody [EPR19089-202] - BSA and Azide free (ab231859)
    ChIP - Anti-MEF2C antibody [EPR19089-202] - BSA and Azide free (ab231859)

    Chromatin was prepared from HUVEC cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with: 25 µg of chromatin, 5 µg of ab211493 (blue), and 20 µl of protein A/G sepharose beads slurry (10 µl of sepharose A beads + 10 µl of sepharose G beads). 5 μg of rabbit normal IgG was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (SYBR green chemistry).

    ChIP was performed according to the literature (PMID: 26923194).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab211493).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2C antibody [EPR19089-202] - BSA and Azide free (ab231859)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2C antibody [EPR19089-202] - BSA and Azide free (ab231859)

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue labelling MEF2C with ab211493 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Nuclear staining in B lymphocytes of rat spleen but not in T cells in the periarterial lymphatic sheath is observed (PMID: 8506376, PMID 15703219). Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP)ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab211493).

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2C antibody [EPR19089-202] - BSA and Azide free (ab231859)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2C antibody [EPR19089-202] - BSA and Azide free (ab231859)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labelling MEF2C with ab211493 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Nuclear staining in B lymphocytes of mouse spleen but not in T cells in the periarterial lymphatic sheath is observed (PMID: 8506376, PMID 15703219). Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP)ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab211493).

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2C antibody [EPR19089-202] - BSA and Azide free (ab231859)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2C antibody [EPR19089-202] - BSA and Azide free (ab231859)

    Immunohistochemical analysis of paraffin-embedded human endometrial carcinoma tissue labelling MEF2C with ab211493 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Nuclear staining in leukocytes but not in tumor cells of human endometrial carcinoma is observed (PMID: 8506376, PMID 15703219). Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP)ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab211493).

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-MEF2C antibody [EPR19089-202] - BSA and Azide free (ab231859)
    Immunocytochemistry/ Immunofluorescence - Anti-MEF2C antibody [EPR19089-202] - BSA and Azide free (ab231859)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilised Raji (human Burkitt's lymphoma B lymphocyte) cells labelling MEF2C with ab211493 at 1/500 dilution, followed by AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution (green). Confocal image showing nuclear staining in Raji cell line. Negative control: Jurkat (PMID: 27876533). DAPI was used as the nuclear counterstain, and the Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889 antibody was used as a counterstain at 1/200 dilution.

    The negetive controls are as follows:
    -ve control 1: ab197070 on jurkat (human T cell leukemia cell line from peripheral blood) cells.
    -ve control 2: Jurkat cells stained with DAPI.
    -ve control 3: Merged negetive contol images.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab211493).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2C antibody [EPR19089-202] - BSA and Azide free (ab231859)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2C antibody [EPR19089-202] - BSA and Azide free (ab231859)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labelling MEF2C with ab211493 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Nuclear staining in human skeletal muscle cells is observed(PMID: 8506376, PMID 15703219). Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab211493).

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Anti-MEF2C antibody [EPR19089-202] - BSA and Azide free (ab231859)
    Anti-MEF2C antibody [EPR19089-202] - BSA and Azide free (ab231859)

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