Anti-MEF2C antibody (ab227085)
Key features and details
- Rabbit polyclonal to MEF2C
- Suitable for: IHC-P, WB, ICC/IF, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-MEF2C antibody
See all MEF2C primary antibodies -
Description
Rabbit polyclonal to MEF2C -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IF, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Sheep, Guinea pig, Cow, Xenopus laevis, Rhesus monkey
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Immunogen
Recombinant fragment within Human MEF2C (internal sequence). The exact sequence is proprietary.
Database link: Q06413 -
Positive control
- WB: U-87 MG whole cell lysate. IP: K562 whole cell extract. IHC-P: Mouse forebrain tissue; Saos2 xenograft. ICC/IF: HeLa cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-MEF2C antibody (ab227085)Anti-MEF2C antibody (ab227085) at 1/10000 dilution + U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate at 30 µg
Developed using the ECL technique.
Predicted band size: 51 kDa
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Immunoprecipitation - Anti-MEF2C antibody (ab227085)MEF2Cwas immunoprecipitated from K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate with ab227085 at 1/100 dilution. Western blot was performed from the immunoprecipitate using ab227085 at 1/1000 dilution.
Lane 1: Control IgG instead of ab227085 in HeLa whole cell extract.
Lane 2: ab227085 IP in K562 whole cell extract.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2C antibody (ab227085)Paraffin-embedded mouse forebrain tissue stained for MEF2C with ab227085 at 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2C antibody (ab227085)Paraffin-embedded Saos-2 (human osteosarcoma cell line) xenograft tissue stained for MEF2C with ab227085 at 1/500 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-MEF2C antibody (ab227085)4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for MEF2C (green) using ab227085 at 1/1000 dilution in ICC/IF.
The cytoskeleton is stained with phalloidin (red).
