Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

Order now and get it on Tuesday March 09, 2021

Anti-MEF2C antibody [EPR1452(N)] (ab191428)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR1452(N)] to MEF2C
Suitable for: IHC-P, WB
Reacts with: Rat, Human

Product name

Anti-MEF2C antibody [EPR1452(N)]
See all MEF2C primary antibodies
Description

Rabbit monoclonal [EPR1452(N)] to MEF2C
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Rat

Human

WB
Rat

Human

See all applications and species data
Immunogen

This information is proprietary to Abcam and/or its suppliers.
Positive control
- Saos-2, Raji, Human fetal brain and Rat kidney lysates; Human tonsil, Human spleen and Rat spleen tissues.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Rat Human
WB	Rat Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR1452(N)
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2C antibody [EPR1452(N)] (ab191428)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling MEF2C with ab191428 at 1/1000 (0.5 μg/ml) with prediluted ImmunoHistoprobe(Ready to use) HRP Polymer for Rabbit IgG as secondary antibody. Negative control: Using PBS instead of primary ab, Secondary ab:ImmunoHistoprobe(Ready to use) HRP Polymer for Rabbit/Mouse IgG.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-MEF2C antibody [EPR1452(N)] (ab191428)

All lanes : Anti-MEF2C antibody [EPR1452(N)] (ab191428) at 1/1000 dilution

Lane 1 : Saos-2 lysate
Lane 2 : Raji lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 51 kDa
Additional bands at: 50-60 kDa. We are unsure as to the identity of these extra bands.
Western blot - Anti-MEF2C antibody [EPR1452(N)] (ab191428)

Anti-MEF2C antibody [EPR1452(N)] (ab191428) at 1/1000 dilution + Human fetal brain lysate at 20 µg

Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 51 kDa
Additional bands at: 50-60 kDa. We are unsure as to the identity of these extra bands.
Western blot - Anti-MEF2C antibody [EPR1452(N)] (ab191428)

Anti-MEF2C antibody [EPR1452(N)] (ab191428) at 1/1000 dilution + Rat brain lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 51 kDa
Additional bands at: 50-60 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2C antibody [EPR1452(N)] (ab191428)

Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling MEF2C with ab191428 at 1/1000 (0.5 μg/ml) with prediluted ImmunoHistoprobe(Ready to use) HRP Polymer for Rabbit IgG as secondary antibody. Negative control: Using PBS instead of primary ab, Secondary ab: ImmunoHistoprobe(Ready to use) HRP Polymer for Rabbit/Mouse IgG.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2C antibody [EPR1452(N)] (ab191428)

Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling MEF2C with ab191428 at 1/1000 (0.5 μg/ml) with prediluted ImmunoHistoprobe(Ready to use) HRP Polymer for Rabbit IgG as secondary antibody. Negative control: Using PBS instead of primary ab, Secondary ab: ImmunoHistoprobe(Ready to use) HRP Polymer for Rabbit/Mouse IgG.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Anti-MEF2C antibody [EPR1452(N)] (ab191428)

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