Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MEF2C antibody [EPR1452(N)] (ab191428)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1452(N)] to MEF2C
  • Suitable for: IHC-P, WB
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-MEF2C antibody [EPR1452(N)]
    See all MEF2C primary antibodies

  • Description

    Rabbit monoclonal [EPR1452(N)] to MEF2C

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Rat
    Human
    WB
    Rat
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Saos-2, Raji, Human fetal brain and Rat kidney lysates; Human tonsil, Human spleen and Rat spleen tissues.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2C antibody [EPR1452(N)] (ab191428)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2C antibody [EPR1452(N)] (ab191428)

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling MEF2C with ab191428 at 1/1000 (0.5 μg/ml) with prediluted ImmunoHistoprobe(Ready to use) HRP Polymer for Rabbit IgG as secondary antibody. Negative control: Using PBS instead of primary ab, Secondary ab:ImmunoHistoprobe(Ready to use) HRP Polymer for Rabbit/Mouse IgG.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-MEF2C antibody [EPR1452(N)] (ab191428)
    Western blot - Anti-MEF2C antibody [EPR1452(N)] (ab191428)
    All lanes : Anti-MEF2C antibody [EPR1452(N)] (ab191428) at 1/1000 dilution

    Lane 1 : Saos-2 lysate
    Lane 2 : Raji lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 51 kDa
    Additional bands at: 50-60 kDa. We are unsure as to the identity of these extra bands.

  • Western blot - Anti-MEF2C antibody [EPR1452(N)] (ab191428)
    Western blot - Anti-MEF2C antibody [EPR1452(N)] (ab191428)
    Anti-MEF2C antibody [EPR1452(N)] (ab191428) at 1/1000 dilution + Human fetal brain lysate at 20 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 51 kDa
    Additional bands at: 50-60 kDa. We are unsure as to the identity of these extra bands.

  • Western blot - Anti-MEF2C antibody [EPR1452(N)] (ab191428)
    Western blot - Anti-MEF2C antibody [EPR1452(N)] (ab191428)
    Anti-MEF2C antibody [EPR1452(N)] (ab191428) at 1/1000 dilution + Rat brain lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 51 kDa
    Additional bands at: 50-60 kDa. We are unsure as to the identity of these extra bands.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2C antibody [EPR1452(N)] (ab191428)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2C antibody [EPR1452(N)] (ab191428)

    Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling MEF2C with ab191428 at 1/1000 (0.5 μg/ml) with prediluted ImmunoHistoprobe(Ready to use) HRP Polymer for Rabbit IgG as secondary antibody. Negative control: Using PBS instead of primary ab, Secondary ab: ImmunoHistoprobe(Ready to use) HRP Polymer for Rabbit/Mouse IgG.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2C antibody [EPR1452(N)] (ab191428)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2C antibody [EPR1452(N)] (ab191428)

    Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling MEF2C with ab191428 at 1/1000 (0.5 μg/ml) with prediluted ImmunoHistoprobe(Ready to use) HRP Polymer for Rabbit IgG as secondary antibody. Negative control: Using PBS instead of primary ab, Secondary ab: ImmunoHistoprobe(Ready to use) HRP Polymer for Rabbit/Mouse IgG.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-MEF2C antibody [EPR1452(N)] (ab191428)
    Anti-MEF2C antibody [EPR1452(N)] (ab191428)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-MEF2C antibody [EPR1452(N)] (ab191428)

© 2021 Astana Biomed Group LLP. All rights reserved.