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Anti-Integrin alpha V antibody [272-17E6] - BSA and Azide free (ab16821)

Key features and details

  • Mouse monoclonal [272-17E6] to Integrin alpha V - BSA and Azide free
  • Suitable for: Flow Cyt
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-Integrin alpha V antibody [272-17E6] - BSA and Azide free
    See all Integrin alpha V primary antibodies

  • Description

    Mouse monoclonal [272-17E6] to Integrin alpha V - BSA and Azide free

  • Host species

    Mouse

  • Specificity

    Cross reacts with all alpha V integrins.

  • Tested applications

    Suitable for: Flow Cytmore details

  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse

  • Immunogen

    Full length native protein (purified) corresponding to Human Integrin alpha V. Human Placental ?vß3
    Database link: P06756

  • Positive control

    • Flow Cyt: Human melanoma cells and HeLa cells

  • General notes

    This product was changed from ascites to tissue culture supernatant on 17 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties

Images

  • Flow Cytometry - Anti-Integrin alpha V antibody [272-17E6] - BSA and Azide free (ab16821)
    Flow Cytometry - Anti-Integrin alpha V antibody [272-17E6] - BSA and Azide free (ab16821)

    Flow Cytometry analysis of HeLa cells labeling Integrin alpha V with ab16821 at 0.5 µg (red), or with 1 µg of Mouse IgG isotype control.

  • Flow Cytometry - Anti-Integrin alpha V antibody [272-17E6] - BSA and Azide free (ab16821)
    Flow Cytometry - Anti-Integrin alpha V antibody [272-17E6] - BSA and Azide free (ab16821) This image is a courtesy of Anonymous Abreview

    ab16821 staining Integrin alpha V in Human melanoma cells by Flow Cytometery. Samples were prepared by trypsinizing cells, treatment with STI and spun out trypsin, kept on ice during experiment duration. FSC and SSC were used to gate healthy cell population, which excluded PI positive cells. The primary antibody was diluted 1/250 and incubated with sample for 1 hour at 4°C in 2% FCS/0. 1%NaAzide/PBS. An Alexa Fuor® 488-conjugated Goat polyclonal to mouse IgG was used at dilution at 1/500 as secondary antibody.

    This image was generated using the ascites version of the product.

    See Abreview

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