Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-UAP1 antibody [EPR10259] - BSA and Azide free (ab249201)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10259] to UAP1 - BSA and Azide free
  • Suitable for: ICC/IF, IHC-P, Flow Cyt, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-UAP1 antibody [EPR10259] - BSA and Azide free
    See all UAP1 primary antibodies

  • Description

    Rabbit monoclonal [EPR10259] to UAP1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IHC-P, Flow Cyt, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab249201 is the carrier-free version of ab155287. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab249201 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-UAP1 antibody [EPR10259] - BSA and Azide free (ab249201)
    Western blot - Anti-UAP1 antibody [EPR10259] - BSA and Azide free (ab249201)
    All lanes : Anti-UAP1 antibody [EPR10259] (ab155287) at 1/1000 dilution

    Lane 1 : Human testis lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : A549 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 59 kDa



    This data was developed using ab155287, the same antibody clone in a different buffer formulation.

  • Immunocytochemistry - Anti-UAP1 antibody [EPR10259] - BSA and Azide free (ab249201)
    Immunocytochemistry - Anti-UAP1 antibody [EPR10259] - BSA and Azide free (ab249201)

    This data was developed using ab155287, the same antibody clone in a different buffer formulation.

    Immunocytochemistry/Immunofluorescence analysis of HeLa (human cervix adenocarcinoma) labelling UAP1 with purified ab155287 at 1/500. Cells were fixed with 100% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue). Control: PBS only
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UAP1 antibody [EPR10259] - BSA and Azide free (ab249201)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UAP1 antibody [EPR10259] - BSA and Azide free (ab249201)

    This data was developed using ab155287, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue labeling UAP1 with ab155287 at 1/50 dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Flow Cytometry - Anti-UAP1 antibody [EPR10259] - BSA and Azide free (ab249201)
    Flow Cytometry - Anti-UAP1 antibody [EPR10259] - BSA and Azide free (ab249201)
    This data was developed using ab155287, the same antibody clone in a different buffer formulation.Flow cytometric analysis of permeabilized Jurkat cells labeling UAP1 with ab155287 at 1/100 dilution (red) compared with a rabbit IgG negative control (green).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UAP1 antibody [EPR10259] - BSA and Azide free (ab249201)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UAP1 antibody [EPR10259] - BSA and Azide free (ab249201)
    This data was developed using ab155287, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human urinary bladder transitional carcinoma tissue using ab155287 showing +ve staining.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UAP1 antibody [EPR10259] - BSA and Azide free (ab249201)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UAP1 antibody [EPR10259] - BSA and Azide free (ab249201)
    This data was developed using ab155287, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human normal tonsil tissue using ab155287 showing +ve staining.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UAP1 antibody [EPR10259] - BSA and Azide free (ab249201)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UAP1 antibody [EPR10259] - BSA and Azide free (ab249201)
    This data was developed using ab155287, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human normal breast tissue using ab155287 showing +ve staining.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UAP1 antibody [EPR10259] - BSA and Azide free (ab249201)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UAP1 antibody [EPR10259] - BSA and Azide free (ab249201)
    This data was developed using ab155287, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human endometrial carcinoma tissue using ab155287 showing +ve staining.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Anti-UAP1 antibody [EPR10259] - BSA and Azide free (ab249201)
    Anti-UAP1 antibody [EPR10259] - BSA and Azide free (ab249201)

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