Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] - BSA and Azide free (ab227991)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP6072(2)] to 5 Lipoxygenase/5-LO - BSA and Azide free
  • Suitable for: ICC/IF, Flow Cyt, IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] - BSA and Azide free
    See all 5 Lipoxygenase/5-LO primary antibodies

  • Description

    Rabbit monoclonal [EP6072(2)] to 5 Lipoxygenase/5-LO - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Mouse
    IHC-P
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Fetal thymus, K562 and HUVEC lysates. Human prostate and Human tonsil tissue. HUVEC cells.

  • General notes

    Ab227991 is the carrier-free version of ab169755. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab227991 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Images

  • Flow Cytometry - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] - BSA and Azide free (ab227991)
    Flow Cytometry - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] - BSA and Azide free (ab227991)

    ab169755 staining 5 Lipoxygenase/5-LO in HUVEC (human umbilical vein/vascular endothelium) by flow cytometry. Cells were fixed with 4% paraformaldehyde, permabilised with 90% methanol and the sample was incubated with the primary antibody at a dilution of 1/130. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.

    Isoytype control: Rabbit monoclonal IgG (black).

    Unlabelled control: Cell without incubation with primary antibody and secondary antibody (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab169755).

  • Immunocytochemistry/ Immunofluorescence - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] - BSA and Azide free (ab227991)
    Immunocytochemistry/ Immunofluorescence - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] - BSA and Azide free (ab227991)

    ab169755 staining 5 Lipoxygenase/5-LO in the Raw264.7 cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody (1/100). ab150077 (1/500) an Alexa Fluor® 488-conjugated Goat anti-rabbit IgG was used as the secondary antibody. Nuclei were counterstained with DAPI.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab169755).

  • Immunocytochemistry/ Immunofluorescence - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] - BSA and Azide free (ab227991)
    Immunocytochemistry/ Immunofluorescence - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] - BSA and Azide free (ab227991)

    Immunofluorescent analysis of HUVEC cells labeling 5 Lipoxygenase/5-LO with ab169755, unpurified, at 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab169755).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] - BSA and Azide free (ab227991)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] - BSA and Azide free (ab227991)

    Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling 5 Lipoxygenase/5-LO with ab169755, unpurified, at 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab169755).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] - BSA and Azide free (ab227991)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] - BSA and Azide free (ab227991)

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling 5 Lipoxygenase/5-LO with ab169755, unpurified, at 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab169755).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] - BSA and Azide free (ab227991)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] - BSA and Azide free (ab227991)

    This IHC data was generated using the same anti-5 Lipoxygenase/5-LO antibody clone, EP6072(2), in a different buffer formulation (ab169755).

    ab169755 staining 5 Lipoxygenase/5-LO in Human tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed and paraffin-embedded, antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Samples were incubated with primary antibody (1/100). An undiluted HRP-conjugated mouse anti-rabbit IgG was used as the secondary antibody. Tissue counterstained with Hematoxylin. PBS was used in the negative control rather than the Primary antibody.

  • Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] - BSA and Azide free (ab227991)
    Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] - BSA and Azide free (ab227991)

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