Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-LAG-3 antibody [EPR20294-96] - BSA and Azide free (ab251584)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20294-96] to LAG-3 - BSA and Azide free
  • Suitable for: Flow Cyt
  • Reacts with: Mouse

Overview

  • Product name

    Anti-LAG-3 antibody [EPR20294-96] - BSA and Azide free
    See all LAG-3 primary antibodies

  • Description

    Rabbit monoclonal [EPR20294-96] to LAG-3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant fragment within Mouse LAG-3 aa 1-250. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: Q61790

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • General notes

    ab251584 is the carrier-free version of ab228411 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab251584 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as Lymphocyte Activation Gene 3.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Flow Cytometry - Anti-LAG-3 antibody [EPR20294-96] - BSA and Azide free (ab251584)
    Flow Cytometry - Anti-LAG-3 antibody [EPR20294-96] - BSA and Azide free (ab251584)
    This data was developed using ab228411, the same antibody clone in a different buffer formulation.Flow cytometric analysis of mouse primary splenocytes treated with 2.5μg/ml concanavalin A for 72h labeling LAG-3 with ab228411 at 1/600 dilution (right) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)(left). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody. Mouse primary splenocytes treated with 2.5μg/ml concanavalin A for 72h, then surface stained with anti-mouse CD3 (Y axis) and ab228411. Only CD3+ population gives a positive signal for Lymphocyte Activation Gene 3. Gated on total viable cells.
  • Anti-LAG-3 antibody [EPR20294-96] - BSA and Azide free (ab251584)
    Anti-LAG-3 antibody [EPR20294-96] - BSA and Azide free (ab251584)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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