Call: +7 771 977 66 65, +7 705 421 2277
Sign in or Register
My basket

Astana Biomed Group, an authorized Abcam distributor in Central Asia

Abiomed homepage

  • Categories
    Signal Transduction
    Cancer
    Epigenetics and Nuclear Signaling
    Immunology
    Cell Biology
    Cardiovascular
    Neuroscience
    Tags & Cell Markers
    Kits/ Lysates/ Other
    Developmental Biology
    Microbiology
    Biochemicals
    Secondary antibodies
    Isotype/Loading Controls
    Antibody Arrays
  • About us
  • Partners
  • Contact
    Address

    Saryarka 32, 18, 010000, Astana city, Kazakhstan

    Telephone +7 771 977 66 65, +7 705 421 2277

    Email

    laboratory@ctlab.kz, orders@abiomed.kz

Back to category
Signal Transduction Protein Trafficking Nuclear Import / Export

Anti-Importin4/Imp4 antibody [EPR13660] - BSA and Azide free (ab250306)

Anti-Importin4/Imp4 antibody [EPR13660] - BSA and Azide free (ab250306)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13660] to Importin4/Imp4 - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), IP, ICC/IF, WB, IHC-P
  • Knockout validated
  • Reacts with: Human

You may also be interested in

Product image
Anti-ABCB4 antibody [EPR23697-35] - BSA and Azide free (ab272459)
Product image
Anti-TPR antibody (ab264153)
Product image
Recombinant Human MOG1 protein (ab130051)
Product image
Anti-MVP antibody [1032] - BSA and Azide free (ab213122)

Overview

  • Product name

    Anti-Importin4/Imp4 antibody [EPR13660] - BSA and Azide free
    See all Importin4/Imp4 primary antibodies
  • Description

    Rabbit monoclonal [EPR13660] to Importin4/Imp4 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), IP, ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment within Human Importin4/Imp4. The exact sequence is proprietary.
    Database link: Q8TEX9

  • Positive control

    • WB: HepG2, HEK293T and A549 cell lysates.
  • General notes

    ab250306 is the carrier-free version of ab181046.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR13660
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Trafficking
    • Nuclear Import / Export
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • Nuclear Receptors
    • Nuclear Pore Complex

Images

  • Western blot - Anti-Importin4/Imp4 antibody [EPR13660] - BSA and Azide free (ab250306)
    Western blot - Anti-Importin4/Imp4 antibody [EPR13660] - BSA and Azide free (ab250306)
    All lanes : Anti-Importin4/Imp4 antibody [EPR13660] (ab181046) at 1/1000 dilution

    Lane 1 : Wild-type HEK293T cell lysate
    Lane 2 : IPO4 knockout HEK293T cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : A549 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 119 kDa
    Observed band size: 130 kDa
    why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation (ab181046).

    Lanes 1-4: Merged signal (red and green). Green - ab181046 observed at 130 kDa. Red - loading control ab8245 observed at 36 kDa. 

     ab181046 Anti-Importin4/Imp4 antibody [EPR13660] was shown to specifically react with Importin4/Imp4 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266175 (knockout cell lysate ab258005) was used.  Wild-type and Importin4/Imp4 knockout samples were subjected to SDS-PAGE.  ab181046 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively.  Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Anti-Importin4/Imp4 antibody [EPR13660] - BSA and Azide free (ab250306)
    Anti-Importin4/Imp4 antibody [EPR13660] - BSA and Azide free (ab250306)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Clear all

Recently viewed products

  •  
  • Product image

    Anti-HOXA9 antibody - C-terminal (ab191178)

  •  
  • Product image

    HRP Anti-Tryptophan Hydroxylase/TPH antibody [EP1311Y] (ab237450)

  •  
  • Product image

    Anti-Luteinizing Hormone beta antibody (ab180787)

Get resources and offers direct to your inbox Sign up
© 2021 Astana Biomed Group LLP. All rights reserved.