Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Ly-6K antibody [EPR23021-61] - BSA and Azide free (ab272394)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23021-61] to Ly-6K - BSA and Azide free
  • Suitable for: IHC-P, WB, IP
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-Ly-6K antibody [EPR23021-61] - BSA and Azide free
    See all Ly-6K primary antibodies

  • Description

    Rabbit monoclonal [EPR23021-61] to Ly-6K - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, IPmore details
    Unsuitable for: Flow Cyt or ICC/IF

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa cell lysate; Human testis tissue lysate. IHC-P:Human testis tissue. IP: Human testis tissue lysate.

  • General notes

    ab272394 is the carrier-free version of ab246486. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ly-6K antibody [EPR23021-61] - BSA and Azide free (ab272394)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ly-6K antibody [EPR23021-61] - BSA and Azide free (ab272394)

    Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling Ly-6K with ab246486 at 1/2000 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in spermatocytes of human testis (PMID:18089789). The section was incubated with ab246486 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab246486).

  • Western blot - Anti-Ly-6K antibody [EPR23021-61] - BSA and Azide free (ab272394)
    Western blot - Anti-Ly-6K antibody [EPR23021-61] - BSA and Azide free (ab272394)
    All lanes : Anti-Ly-6K antibody [EPR23021-61] (ab246486) at 1/1000 dilution

    Lane 1 : Wild-type HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : LY6K knockout HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 19 kDa
    Observed band size: 24 kDa
    why is the actual band size different from the predicted?



    This data was developed using 246486, the same antibody clone in a different buffer formulation.

    Lanes 1-2: Merged signal (red and green). Green - ab246486 observed at 24 kDa. Red - loading control ab8245 observed at 36 kDa.

     ab246486 Anti-Ly-6K antibody [EPR23021-61]  was shown to specifically react with Ly-6K in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265874 (knockout cell lysate ab263248) was used. Wild-type and Ly-6K knockout samples were subjected to SDS-PAGE. ab246486 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunoprecipitation - Anti-Ly-6K antibody [EPR23021-61] - BSA and Azide free (ab272394)
    Immunoprecipitation - Anti-Ly-6K antibody [EPR23021-61] - BSA and Azide free (ab272394)

    Ly-6K was immunoprecipitated from 0.35 mg Human testis tissue lysate with ab246486 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab246486 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/1000 dilution.

    Lane 1: Human testis tissue lysate 10 ug

    Lane 2: ab246486 IP in Human testis tissue lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab246486 in human testis tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab246486).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ly-6K antibody [EPR23021-61] - BSA and Azide free (ab272394)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ly-6K antibody [EPR23021-61] - BSA and Azide free (ab272394)

    Immunohistochemical analysis of paraffin-embedded Human heart tissue labeling Ly-6K with ab246486 at 1/2000 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Negative control: No staining in human heart (PMID:18089789). The section was incubated with ab246486 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab246486).

  • Anti-Ly-6K antibody [EPR23021-61] - BSA and Azide free (ab272394)
    Anti-Ly-6K antibody [EPR23021-61] - BSA and Azide free (ab272394)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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