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Anti-Sialoadhesin/CD169 antibody [SP213] - C-terminal (ab227672)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP213] to Sialoadhesin/CD169 - C-terminal
  • Suitable for: Flow Cyt, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Sialoadhesin/CD169 antibody [SP213] - C-terminal
    See all Sialoadhesin/CD169 primary antibodies

  • Description

    Rabbit monoclonal [SP213] to Sialoadhesin/CD169 - C-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human liver, bone marrow, colon, lung, and adrenal gland tissue; Flow Cyt: Human PBMC treated with 20ng/ml IFN-gamma for 24 hours cells.

  • General notes

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

     This product was previously labelled as Sialoadhesin

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.60
    Preservative: 0.1% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...

  • Purity

    Protein A/G purified

  • Purification notes

    Purified from TCS by protein A/G.

  • Clonality

    Monoclonal

  • Clone number

    SP213

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sialoadhesin/CD169 antibody [SP213] - C-terminal (ab227672)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sialoadhesin/CD169 antibody [SP213] - C-terminal (ab227672)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon tissue sections labeling Sialoadhesin/CD169 with ab227672 at 1/100 dilution (1.17 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sialoadhesin/CD169 antibody [SP213] - C-terminal (ab227672)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sialoadhesin/CD169 antibody [SP213] - C-terminal (ab227672)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver tissue sections labeling Sialoadhesin/CD169 with ab227672 at 1/100 dilution (1.17 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Flow Cytometry - Anti-Sialoadhesin/CD169 antibody [SP213] - C-terminal (ab227672)
    Flow Cytometry - Anti-Sialoadhesin/CD169 antibody [SP213] - C-terminal (ab227672)

    Flow Cytometry analysis of Human PBMC treated with 20ng/ml IFN-gamma for 24 hours cells labeling Sialoadhesin/CD169 with purified ab227672 at 1/20 dilution (6 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.1% Tween-20. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730). Unlabeled control - Unlabelled cells.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sialoadhesin/CD169 antibody [SP213] - C-terminal (ab227672)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sialoadhesin/CD169 antibody [SP213] - C-terminal (ab227672)

    Formalin-fixed, paraffin-embedded human liver tissue stained for Sialoadhesin/CD169 with ab227672 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sialoadhesin/CD169 antibody [SP213] - C-terminal (ab227672)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sialoadhesin/CD169 antibody [SP213] - C-terminal (ab227672)

    Formalin-fixed, paraffin-embedded human bone marrow tissue stained for Sialoadhesin/CD169 with ab227672 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sialoadhesin/CD169 antibody [SP213] - C-terminal (ab227672)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sialoadhesin/CD169 antibody [SP213] - C-terminal (ab227672)

    Formalin-fixed, paraffin-embedded human colon tissue stained for Sialoadhesin/CD169 with ab227672 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sialoadhesin/CD169 antibody [SP213] - C-terminal (ab227672)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sialoadhesin/CD169 antibody [SP213] - C-terminal (ab227672)

    Formalin-fixed, paraffin-embedded human lung tissue stained for Sialoadhesin/CD169 with ab227672 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sialoadhesin/CD169 antibody [SP213] - C-terminal (ab227672)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sialoadhesin/CD169 antibody [SP213] - C-terminal (ab227672)

    Formalin-fixed, paraffin-embedded human adrenal gland tissue stained for Sialoadhesin/CD169 with ab227672 at 1/100 dilution in immunohistochemical analysis.

  • Anti-Sialoadhesin/CD169 antibody [SP213] - C-terminal (ab227672)
    Anti-Sialoadhesin/CD169 antibody [SP213] - C-terminal (ab227672)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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