Anti-Sialoadhesin/CD169 antibody [SP213] - C-terminal (ab227672)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP213] to Sialoadhesin/CD169 - C-terminal
- Suitable for: Flow Cyt, IHC-P
- Reacts with: Human
Overview
-
Product name
Anti-Sialoadhesin/CD169 antibody [SP213] - C-terminal
See all Sialoadhesin/CD169 primary antibodies -
Description
Rabbit monoclonal [SP213] to Sialoadhesin/CD169 - C-terminal -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- IHC-P: Human liver, bone marrow, colon, lung, and adrenal gland tissue; Flow Cyt: Human PBMC treated with 20ng/ml IFN-gamma for 24 hours cells.
-
General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
This product was previously labelled as Sialoadhesin
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
-
Purity
Protein A/G purified -
Purification notes
Purified from TCS by protein A/G. -
Clonality
Monoclonal -
Clone number
SP213 -
Isotype
IgG -
Research areas
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon tissue sections labeling Sialoadhesin/CD169 with ab227672 at 1/100 dilution (1.17 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver tissue sections labeling Sialoadhesin/CD169 with ab227672 at 1/100 dilution (1.17 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
-
Flow Cytometry analysis of Human PBMC treated with 20ng/ml IFN-gamma for 24 hours cells labeling Sialoadhesin/CD169 with purified ab227672 at 1/20 dilution (6 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.1% Tween-20. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730). Unlabeled control - Unlabelled cells.
-
Formalin-fixed, paraffin-embedded human liver tissue stained for Sialoadhesin/CD169 with ab227672 at 1/100 dilution in immunohistochemical analysis.
-
Formalin-fixed, paraffin-embedded human bone marrow tissue stained for Sialoadhesin/CD169 with ab227672 at 1/100 dilution in immunohistochemical analysis.
-
Formalin-fixed, paraffin-embedded human colon tissue stained for Sialoadhesin/CD169 with ab227672 at 1/100 dilution in immunohistochemical analysis.
-
Formalin-fixed, paraffin-embedded human lung tissue stained for Sialoadhesin/CD169 with ab227672 at 1/100 dilution in immunohistochemical analysis.
-
Formalin-fixed, paraffin-embedded human adrenal gland tissue stained for Sialoadhesin/CD169 with ab227672 at 1/100 dilution in immunohistochemical analysis.
-