Human IL-5 ELISA Kit (ab215536)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 3.3 pg/ml
- Range: 4.7 pg/ml - 300 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human IL-5 ELISA Kit
See all IL-5 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 5 8.6% Inter-assay Sample n Mean SD CV% Overall 3 5.4% -
Sample type
Cell culture supernatant, Urine, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
3.3 pg/ml -
Range
4.7 pg/ml - 300 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 105 104% - 107% Urine 81 80% - 81% Serum 96 93% - 100% Hep Plasma 99 97% - 100% EDTA Plasma 94 93% - 96% Cit plasma 93 90% - 96% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human
Does not react with: Cow -
Product overview
Human IL-5 ELISA Kit (ab215536) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-5 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IL-5 with 3.3 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Interleukin 5 (IL-5) is a small, 113 amino acid cytokine produced by T cells involved in the differentiation of B-cells to immunoglobulin secreting cells. IL-5 is a key mediator in the survival and effector functions of mature eosinophils. Due to its effects on eosinophils, IL-5 is implicated in the pathogenesis of asthma and other hypereosinophilic inflammatory conditions.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human IL-5 Capture Antibody 1 x 600µl 10X Human IL-5 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Human IL-5 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Factor that induces terminal differentiation of late-developing B-cells to immunoglobulin secreting cells. -
Sequence similarities
Belongs to the IL-5 family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- B-cell differentiation factor I
- Colony stimulating factor
- EDF
see all -
Database links
- Entrez Gene: 3567 Human
- Omim: 147850 Human
- SwissProt: P05113 Human
- Unigene: 2247 Human
Images
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Other - Human IL-5 ELISA Kit (ab215536)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Human IL-5 standard curve comparison data.Standard curve comparison between human IL-5 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows comparable sensitivity with shorter protocol time.
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Example of human IL-5 standard curve in Sample Diluent NS.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of spiked IL-5 in human serum, plasma and urine samples.The concentrations of IL-5 were measured in duplicates, interpolated from the IL-5 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (heparin) 50% and urine 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-5 concentration was determined to be 149 pg/mL in serum, 131 pg/mL in plasma (citrate), 165 pg/mL in plasma (heparin), 142 pg/mL in plasma (EDTA) and 133 pg/mL in urine.
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Human peripheral blood mononuclear cells were cultured unstimulated or stimulated with 10 µg/mL PHA.Conditioned media was harvested after 48 hours. IL-5 was measured in 100% unstimulated and PHA stimulated PBMC supernatant. The concentrations of IL-5 were measured in duplicate, interpolated from the IL-5 standard curves and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-5 concentration was determined to be 226.9 pg/mL in PHA stimulated PBMC supernatant and 1.94 pg/mL in unstimulated PBMC supernatant.
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Sandwich ELISA - Human IL-5 ELISA Kit (ab215536)To learn more about the advantages of recombinant antibodies see here.
