Antibodies, Proteins, Kits and Reagents for Life Science

278 083 ₸ Product size

100 µl 278 083 ₸

Order now and get it on Wednesday March 10, 2021

Anti-BANK1 antibody [EPR20788] (ab229190)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR20788] to BANK1
Suitable for: IHC-P, Flow Cyt
Reacts with: Human

Product name

Anti-BANK1 antibody [EPR20788]
See all BANK1 primary antibodies
Description

Rabbit monoclonal [EPR20788] to BANK1
Host species

Rabbit
Tested Applications & Species

Application Species
Flow Cyt
Human

IHC-P
Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- IHC-P: Human tonsil tissue; Human Large B cell lymphoma tissue. Flow Cytometry: Daudi cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
Flow Cyt	Human
IHC-P	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR20788
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BANK1 antibody [EPR20788] (ab229190)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling BANK1 with ab229190 at 1/4000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining is weak in the germinal center but strong in the mantle zones of human tonsil (PMID: 24879116). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BANK1 antibody [EPR20788] (ab229190)

Immunohistochemical analysis of paraffin-embedded human Large B cell lymphoma tissue labeling BANK1 with ab229190 at 1/4000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining in human Large B Cell Lymphoma cells (PMID: 24879116) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Flow Cytometry - Anti-BANK1 antibody [EPR20788] (ab229190)

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized Jurkat (Human T cell leukemia T lymphocyte, Left panel) / Daudi (human Burkitt's lymphoma lymphoblast, Right panel) cell lines labeling BANK1 with ab229190 at 1/400 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

The Jurkat cell line is a negative control for BANK1.
Anti-BANK1 antibody [EPR20788] (ab229190)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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