Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-BANK1 antibody [EPR20788] (ab229190)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20788] to BANK1
  • Suitable for: IHC-P, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-BANK1 antibody [EPR20788]
    See all BANK1 primary antibodies

  • Description

    Rabbit monoclonal [EPR20788] to BANK1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human tonsil tissue; Human Large B cell lymphoma tissue. Flow Cytometry: Daudi cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR20788

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BANK1 antibody [EPR20788] (ab229190)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BANK1 antibody [EPR20788] (ab229190)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling BANK1 with ab229190 at 1/4000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining is weak in the germinal center but strong in the mantle zones of human tonsil (PMID: 24879116). Counter stained with Hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BANK1 antibody [EPR20788] (ab229190)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BANK1 antibody [EPR20788] (ab229190)

    Immunohistochemical analysis of paraffin-embedded human Large B cell lymphoma tissue labeling BANK1 with ab229190 at 1/4000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining in human Large B Cell Lymphoma cells (PMID: 24879116) is observed. Counter stained with Hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Flow Cytometry - Anti-BANK1 antibody [EPR20788] (ab229190)
    Flow Cytometry - Anti-BANK1 antibody [EPR20788] (ab229190)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized Jurkat (Human T cell leukemia T lymphocyte, Left panel) / Daudi (human Burkitt's lymphoma lymphoblast, Right panel) cell lines labeling BANK1 with ab229190 at 1/400 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

    The Jurkat cell line is a negative control for BANK1.

  • Anti-BANK1 antibody [EPR20788] (ab229190)
    Anti-BANK1 antibody [EPR20788] (ab229190)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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