Anti-BACH2 antibody [7A4] (ab243148)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [7A4] to BACH2
- Suitable for: WB
- Reacts with: Mouse, Human
Overview
-
Product name
Anti-BACH2 antibody [7A4]
See all BACH2 primary antibodies -
Description
Rat monoclonal [7A4] to BACH2 -
Host species
Rat -
Tested Applications & Species
See all applications and species dataApplication Species WB Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: HEK-293T, Ramos, NAMALWA and WEHI-231 whole cell lysate.
-
General notes
This antibody clone is manufactured by Abcam. If you require a different buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
Purity
Ion Exchange Chromatography -
Clonality
Monoclonal -
Clone number
7A4 -
Isotype
IgG2a -
Research areas
Images
-
All lanes : Anti-BACH2 antibody [7A4] (ab243148) at 0.502 µg/ml
Lane 1 : 293T (human embryonic kidney epithelial cell), whole cell lysate
Lane 2 : Ramos (human Burkitt's lymphoma B lymphocyte), whole cell lysate
Lane 3 : THP-1 (human monocytic leukemia monocyte), whole cell lysate
Lane 4 : HL-60 (human Acute Promyelocytic Leukemia promyeloblast), whole cell lysate
Lane 5 : NAMALWA (human Burkitt's lymphoma B lymphocyte), whole cell lysate
Lane 6 : WEHI-231 (mouse B cell lymphoma B lymphocyte), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution
Predicted band size: 93 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?Blocking/diluting buffer and concentration: 5% NFDM/TBST.
Exposure time:
Lane 1: 3 minutes
Lanes 2-5: 3 seconds
Lane 6: 5 secondsNegative control: THP-1 and HL-60 (PMID: 10949928).
The molecular weight observed is consistent with what has been described in the literature (PMID: 9755173).
-