Anti-TPD52 antibody [EPR14219] - BSA and Azide free (ab250473)
![Anti-TPD52 antibody [EPR14219] - BSA and Azide free (ab250473)](https://www.abcam.com/ps/products/250/ab250473/Images/ab250473-446452-anti-tpd52-antibody-epr14219-bsa-and-azide-free-western-blot.jpg "Anti-TPD52 antibody [EPR14219] - BSA and Azide free (ab250473)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14219] to TPD52 - BSA and Azide free
- Suitable for: IHC-P, Flow Cyt, ICC/IF, WB
- Reacts with: Mouse, Human
Overview
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Product name
Anti-TPD52 antibody [EPR14219] - BSA and Azide free
See all TPD52 primary antibodies -
Description
Rabbit monoclonal [EPR14219] to TPD52 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, Flow Cyt, ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab250473 is the carrier-free version of ab181260. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab250473 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR14219 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-TPD52 antibody [EPR14219] - BSA and Azide free (ab250473)All lanes : Anti-TPD52 antibody [EPR14219] (ab181260) at 1/2000 dilution
Lane 1 : MCF7 cell lysate
Lane 2 : THP1 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 24 kDaThis data was developed using ab181260, the same antibody clone in a different buffer formulation.
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![Immunocytochemistry - Anti-TPD52 antibody [EPR14219] - BSA and Azide free (ab250473)](https://www.abcam.com/ps/products/250/ab250473/Images/ab250473-6-anti-tpd52-antibody-epr14219-bsa-and-azide-free-immunocytochemistry.jpg)
Immunocytochemistry - Anti-TPD52 antibody [EPR14219] - BSA and Azide free (ab250473)This data was developed using ab181260, the same antibody clone in a different buffer formulation.
Immunocytochemistry/Immunofluorescence analysis of THP-1 cells labelling TPD52 with ab181260 at 1/1000. Cells were fixed with 100% Paraformaldehyde. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.
Control: PBS only.
Nuclear counter stain: DAPI. -
![Western blot - Anti-TPD52 antibody [EPR14219] - BSA and Azide free (ab250473)](https://www.abcam.com/ps/products/250/ab250473/Images/ab250473-410328-anti-tpd52-antibody-epr14219-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-TPD52 antibody [EPR14219] - BSA and Azide free (ab250473) -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPD52 antibody [EPR14219] - BSA and Azide free (ab250473)](https://www.abcam.com/ps/products/250/ab250473/Images/ab250473-3-anti-tpd52-antibody-epr14219-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPD52 antibody [EPR14219] - BSA and Azide free (ab250473)This data was developed using ab181260, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human Kidney tissue labeling TPD52 with ab181260 at 1/100 dilution, followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPD52 antibody [EPR14219] - BSA and Azide free (ab250473)](https://www.abcam.com/ps/products/250/ab250473/Images/ab250473-2-anti-tpd52-antibody-epr14219-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPD52 antibody [EPR14219] - BSA and Azide free (ab250473)This data was developed using ab181260, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human Liver tissue labeling TPD52 with ab181260 at 1/100 dilution, followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol. -
![Flow Cytometry - Anti-TPD52 antibody [EPR14219] - BSA and Azide free (ab250473)](https://www.abcam.com/ps/products/250/ab250473/Images/ab250473-1-anti-tpd52-antibody-epr14219-bsa-and-azide-free-flow-cytometry.jpg)
Flow Cytometry - Anti-TPD52 antibody [EPR14219] - BSA and Azide free (ab250473)This data was developed using ab181260, the same antibody clone in a different buffer formulation.Flow cytometric analysis of MCF7 cells (2% paraformaldehyde-fixed) labeling TPD52 with ab181260 at 1/10 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution. -
![Anti-TPD52 antibody [EPR14219] - BSA and Azide free (ab250473)](https://www.abcam.com/ps/products/250/ab250473/Images/ab250473-5-benefits-of-recombinant-antibodies.png)
Anti-TPD52 antibody [EPR14219] - BSA and Azide free (ab250473)