Anti-Glutamine Synthetase antibody [EPR16661] - BSA and Azide free (ab251231)
![Anti-Glutamine Synthetase antibody [EPR16661] - BSA and Azide free (ab251231)](https://www.abcam.com/ps/products/251/ab251231/Images/ab251231-393441-anti-glutamine-synthetase-antibody-epr16661-western-blot-hela-lysates.jpg "Anti-Glutamine Synthetase antibody [EPR16661] - BSA and Azide free (ab251231)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16661] to Glutamine Synthetase - BSA and Azide free
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Human
Overview
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Product name
Anti-Glutamine Synthetase antibody [EPR16661] - BSA and Azide free
See all Glutamine Synthetase primary antibodies -
Description
Rabbit monoclonal [EPR16661] to Glutamine Synthetase - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa cell lysate.
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General notes
Ab251231 is the carrier-free version of ab197024. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab251231 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
EPR16661 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Glutamine Synthetase antibody [EPR16661] - BSA and Azide free (ab251231)All lanes : Anti-Glutamine Synthetase antibody [EPR16661] (ab197024) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : GLUL knockout HeLa cell lysate
Lysates/proteins at 40 µg per lane.
Performed under reducing conditions.
Predicted band size: 42 kDa
Observed band size: 42 kDaThis data was developed using the same antibody clone in a different buffer formation (ab197024).
Lanes 1- 2: Merged signal (red and green). Green - ab197024 observed at 42 kDa. Red - Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) observed at 50 kDa.
ab197024 was shown to react with Glutamine Synthetase in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab261737 (knockout cell lysate ab256930) was used. Wild-type HeLa and GLUL knockout HeLa cell lysates were subjected to SDS-PAGE. ab197024 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Anti-Glutamine Synthetase antibody [EPR16661] - BSA and Azide free (ab251231)](https://www.abcam.com/ps/products/251/ab251231/Images/ab251231-2-benefits-of-recombinant-antibodies.png)
Anti-Glutamine Synthetase antibody [EPR16661] - BSA and Azide free (ab251231)
