Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PNK/PNKP antibody [EPR11935] - BSA and Azide free (ab250354)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11935] to PNK/PNKP - BSA and Azide free
  • Suitable for: IHC-P, IP, WB
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-PNK/PNKP antibody [EPR11935] - BSA and Azide free
    See all PNK/PNKP primary antibodies

  • Description

    Rabbit monoclonal [EPR11935] to PNK/PNKP - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, IP, WBmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab250354 is the carrier-free version of ab181107 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab250354 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as PNK

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-PNK/PNKP antibody [EPR11935] - BSA and Azide free (ab250354)
    Western blot - Anti-PNK/PNKP antibody [EPR11935] - BSA and Azide free (ab250354)
    All lanes : Anti-PNK/PNKP antibody [EPR11935] - C-terminal (ab181107) at 1/2000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : Human testis tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 57 kDa
    Observed band size: 60 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab181107, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11935] - BSA and Azide free (ab250354)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11935] - BSA and Azide free (ab250354)
    This data was developed using ab181107, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling PNK/PNKP with ab181107 at 1/100 dilution. Counter stain Hematoxylin. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11935] - BSA and Azide free (ab250354)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11935] - BSA and Azide free (ab250354)
    This data was developed using ab181107, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling PNK/PNKP with ab181107 at 1/100 dilution. Counter stain Hematoxylin. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Western blot - Anti-PNK/PNKP antibody [EPR11935] - BSA and Azide free (ab250354)
    Western blot - Anti-PNK/PNKP antibody [EPR11935] - BSA and Azide free (ab250354)
    All lanes : Anti-PNK/PNKP antibody [EPR11935] - C-terminal (ab181107) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : PC-3 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 57 kDa
    Observed band size: 60 kDa why is the actual band size different from the predicted?



    This data was developed using ab181107, the same antibody clone in a different buffer formulation.

  • Immunoprecipitation - Anti-PNK/PNKP antibody [EPR11935] - BSA and Azide free (ab250354)
    Immunoprecipitation - Anti-PNK/PNKP antibody [EPR11935] - BSA and Azide free (ab250354)
    This data was developed using ab181107, the same antibody clone in a different buffer formulation.Western blot analysis of PNK/PNKP in immunoprecipitation pellets from HeLa cell lysate. ab181107 used at 1/20 dilution. Secondary antibody Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500. Right lane shows negative control.
  • Anti-PNK/PNKP antibody [EPR11935] - BSA and Azide free (ab250354)
    Anti-PNK/PNKP antibody [EPR11935] - BSA and Azide free (ab250354)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Clear all

Recently viewed products

© 2021 Astana Biomed Group LLP. All rights reserved.