Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Lumican antibody [EPR22510-47] (ab252924)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22510-47] to Lumican
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Lumican antibody [EPR22510-47]
    See all Lumican primary antibodies

  • Description

    Rabbit monoclonal [EPR22510-47] to Lumican

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Mouse
    Rat
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human colon and kidney tissue. Mouse skin tissue. Rat colon tissue. WB: Mouse and rat skin tissue lysate. Saos-2 whole cell lysate. Human colon, skin and placenta tissue lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Lumican antibody [EPR22510-47] (ab252924)
    Western blot - Anti-Lumican antibody [EPR22510-47] (ab252924)
    All lanes : Anti-Lumican antibody [EPR22510-47] (ab252924) at 1/1000 dilution

    Lane 1 : Human colon tissue lysate
    Lane 2 : Human skin tissue lysate
    Lane 3 : Human placenta tissue lysate
    Lane 4 : Mouse skin tissue lysate
    Lane 5 : Rat skin tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Lanes 1-3 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
    Lanes 4-5 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 38 kDa
    Observed band size: 50,70 kDa
    why is the actual band size different from the predicted?



    Exposure time

    Lane 1: 81 seconds
    Lane 2: 15 seconds
    Lane 3: 3 minutes
    Lanes 4-5: 26 seconds

    Blocking buffer and concentration 5% NFDM/TBST
    Diluting buffer and concentration 5% NFDM/TBST

    The molecular weight observed is consistent with what has been described in the literature (PMID:28459201,22266188,18949819)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lumican antibody [EPR22510-47] (ab252924)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lumican antibody [EPR22510-47] (ab252924)

    Immunohistochemical analysis of paraffin-embedded human colon tissue labeling Lumican with ab252924 at 1/4000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in stroma cells of human colon (PMID:17089042, 23399832) is observed. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Western blot - Anti-Lumican antibody [EPR22510-47] (ab252924)
    Western blot - Anti-Lumican antibody [EPR22510-47] (ab252924)
    All lanes : Anti-Lumican antibody [EPR22510-47] (ab252924) at 1/1000 dilution

    Lane 1 : Human kidney tissue lysate
    Lane 2 : Saos-2 (human osteosarcoma epithelial), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 38 kDa


    Exposure time: 3 minutes


    The molecular weight observed is consistent with what has been described in the literature (PMID:28459201,22266188,18949819).

    This blot was developed using a higher sensitivity ECL substrate.

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lumican antibody [EPR22510-47] (ab252924)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lumican antibody [EPR22510-47] (ab252924)

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue labeling Lumican with ab252924 at 1/4000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in stroma cells of mouse skin (PMID:17089042) is observed. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lumican antibody [EPR22510-47] (ab252924)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lumican antibody [EPR22510-47] (ab252924)

    Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling Lumican with ab252924 at 1/4000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in stroma cells of rat colon (PMID:17089042, 23399832) is observed. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Anti-Lumican antibody [EPR22510-47] (ab252924)
    Anti-Lumican antibody [EPR22510-47] (ab252924)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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