Antibodies, Proteins, Kits and Reagents for Life Science

307 566 ₸ Product size

100 µl 307 566 ₸

Order now and get it on Wednesday March 10, 2021

Anti-Lumican antibody [EPR22510-47] (ab252924)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22510-47] to Lumican
Suitable for: WB, IHC-P
Reacts with: Mouse, Rat, Human

Product name

Anti-Lumican antibody [EPR22510-47]
See all Lumican primary antibodies
Description

Rabbit monoclonal [EPR22510-47] to Lumican
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Mouse Rat Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- IHC-P: Human colon and kidney tissue. Mouse skin tissue. Rat colon tissue. WB: Mouse and rat skin tissue lysate. Saos-2 whole cell lysate. Human colon, skin and placenta tissue lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22510-47
Isotype

IgG
Research areas

Western blot - Anti-Lumican antibody [EPR22510-47] (ab252924)

All lanes : Anti-Lumican antibody [EPR22510-47] (ab252924) at 1/1000 dilution

Lane 1 : Human colon tissue lysate
Lane 2 : Human skin tissue lysate
Lane 3 : Human placenta tissue lysate
Lane 4 : Mouse skin tissue lysate
Lane 5 : Rat skin tissue lysate

Lysates/proteins at 10 µg per lane.

Secondary
Lanes 1-3 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Lanes 4-5 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 38 kDa
Observed band size: 50,70 kDa
why is the actual band size different from the predicted?

Exposure time

Lane 1: 81 seconds
Lane 2: 15 seconds
Lane 3: 3 minutes
Lanes 4-5: 26 seconds

Blocking buffer and concentration 5% NFDM/TBST
Diluting buffer and concentration 5% NFDM/TBST

The molecular weight observed is consistent with what has been described in the literature (PMID:28459201,22266188,18949819)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lumican antibody [EPR22510-47] (ab252924)

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling Lumican with ab252924 at 1/4000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in stroma cells of human colon (PMID:17089042, 23399832) is observed. Counterstained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Western blot - Anti-Lumican antibody [EPR22510-47] (ab252924)

All lanes : Anti-Lumican antibody [EPR22510-47] (ab252924) at 1/1000 dilution

Lane 1 : Human kidney tissue lysate
Lane 2 : Saos-2 (human osteosarcoma epithelial), whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 38 kDa

Exposure time: 3 minutes

The molecular weight observed is consistent with what has been described in the literature (PMID:28459201,22266188,18949819).

This blot was developed using a higher sensitivity ECL substrate.

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lumican antibody [EPR22510-47] (ab252924)

Immunohistochemical analysis of paraffin-embedded mouse skin tissue labeling Lumican with ab252924 at 1/4000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in stroma cells of mouse skin (PMID:17089042) is observed. Counterstained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lumican antibody [EPR22510-47] (ab252924)

Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling Lumican with ab252924 at 1/4000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in stroma cells of rat colon (PMID:17089042, 23399832) is observed. Counterstained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Anti-Lumican antibody [EPR22510-47] (ab252924)

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