Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CDC42 antibody [28-10] - BSA and Azide free (ab264478)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [28-10] to CDC42 - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-CDC42 antibody [28-10] - BSA and Azide free
    See all CDC42 primary antibodies

  • Description

    Mouse monoclonal [28-10] to CDC42 - BSA and Azide free

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Recombinant full length protein within CDC42. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: P19073

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: Jurkat, THP1, PC-12 and NIH/3T3 whole cell lysate. Mouse and rat brain and spleen tissue lysate.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab264478 is the carrier-free version of ab255897. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Western blot - Anti-CDC42 antibody [28-10] - BSA and Azide free (ab264478)
    Western blot - Anti-CDC42 antibody [28-10] - BSA and Azide free (ab264478)
    Lane 1 : Anti-CDC42 antibody [28-10] (ab255897) at 0.544 µg/ml
    Lanes 2-4 : Anti-CDC42 antibody [28-10] (ab255897) at 0.554 µg/ml

    Lane 1 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
    Lane 2 : THP1 (human monocytic leukemia monocyte), whole cell lysate
    Lane 3 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
    Lane 4 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution

    Predicted band size: 21 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1: 5 seconds; Lane 2-4: 26 seconds.

    This image was produced using the same antibody clone but in a different formulation ab255897, PBS, sodium azide, glycerol and BSA.

  • Western blot - Anti-CDC42 antibody [28-10] - BSA and Azide free (ab264478)
    Western blot - Anti-CDC42 antibody [28-10] - BSA and Azide free (ab264478)
    All lanes : Anti-CDC42 antibody [28-10] (ab255897) at 0.554 µg/ml

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Mouse spleen tissue lysate
    Lane 3 : Rat brain tissue lysate
    Lane 4 : Rat spleen tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution

    Predicted band size: 21 kDa


    Exposure time: 3 seconds


    This image was produced using the same antibody clone but in a different formulation ab255897, PBS, sodium azide, glycerol and BSA.

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-CDC42 antibody [28-10] - BSA and Azide free (ab264478)
    Anti-CDC42 antibody [28-10] - BSA and Azide free (ab264478)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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