Anti-CDC42 antibody [28-10] - BSA and Azide free (ab264478)
![Anti-CDC42 antibody [28-10] - BSA and Azide free (ab264478)](https://www.abcam.com/ps/products/264/ab264478/Images/ab264478-369298-AntiCDC42-antibody-2810-western-blot-jurkat-thp1-nih3t3-pc12-human-mouse-rat.jpg "Anti-CDC42 antibody [28-10] - BSA and Azide free (ab264478)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [28-10] to CDC42 - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-CDC42 antibody [28-10] - BSA and Azide free
See all CDC42 primary antibodies -
Description
Mouse monoclonal [28-10] to CDC42 - BSA and Azide free -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRatHuman
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Immunogen
Recombinant full length protein within CDC42. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P19073 -
Positive control
- WB: Jurkat, THP1, PC-12 and NIH/3T3 whole cell lysate. Mouse and rat brain and spleen tissue lysate.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
ab264478 is the carrier-free version of ab255897. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
28-10 -
Isotype
IgG2b -
Research areas
Images
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Western blot - Anti-CDC42 antibody [28-10] - BSA and Azide free (ab264478)Lane 1 : Anti-CDC42 antibody [28-10] (ab255897) at 0.544 µg/ml
Lanes 2-4 : Anti-CDC42 antibody [28-10] (ab255897) at 0.554 µg/ml
Lane 1 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
Lane 2 : THP1 (human monocytic leukemia monocyte), whole cell lysate
Lane 3 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
Lane 4 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution
Predicted band size: 21 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1: 5 seconds; Lane 2-4: 26 seconds.
This image was produced using the same antibody clone but in a different formulation ab255897, PBS, sodium azide, glycerol and BSA.
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![Western blot - Anti-CDC42 antibody [28-10] - BSA and Azide free (ab264478)](https://www.abcam.com/ps/products/264/ab264478/Images/ab264478-369297-AntiCDC42-antibody-2810-western-blot-brain-spleen-mouse-rat.jpg)
Western blot - Anti-CDC42 antibody [28-10] - BSA and Azide free (ab264478)All lanes : Anti-CDC42 antibody [28-10] (ab255897) at 0.554 µg/ml
Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse spleen tissue lysate
Lane 3 : Rat brain tissue lysate
Lane 4 : Rat spleen tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution
Predicted band size: 21 kDa
Exposure time: 3 secondsThis image was produced using the same antibody clone but in a different formulation ab255897, PBS, sodium azide, glycerol and BSA.
Blocking/Dilution buffer: 5% NFDM/TBST.
-
![Anti-CDC42 antibody [28-10] - BSA and Azide free (ab264478)](https://www.abcam.com/ps/products/264/ab264478/Images/ab264478-3-benefits-of-recombinant-antibodies.png)
Anti-CDC42 antibody [28-10] - BSA and Azide free (ab264478)
